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pAAVS1-Puro-CAG-mNG2(1-10)

Plasmid

#206042

Purpose

Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

Depositor

Alisa Piekny

Article

Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

Insert

AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette

Use

CRISPR and Synthetic Biology; Donor template

Expression

Mammalian

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mScarlet-LAMP1

Plasmid

#185138

Purpose

Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

Depositor

Andrew York

Article

Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

Insert

Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)

Tags

mScarlet

Expression

Mammalian

Mutation

deleted amino acids 1-21 of LAMP1

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBMN-YFP-Parkin

Plasmid

#59416

Purpose

to make YFP-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

YFP-Parkin (PRKN Human)

Use

Retroviral

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Atl1

Plasmid

#86678

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL1 (ATL1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-CaMKII-TVA-P2A-N2cG

Plasmid

#172362

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKrox24(2xD-E)DsRed

Plasmid

#200111

Purpose

Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

modified EGR1 promoter

Expression

Mammalian

Mutation

highly modified sequence

Promoter

2 copies of D-E element, no other promoter elemen…

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

Plasmid

#84471

Purpose

pAAV-RAM-ChR2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

Channelrhodopsin 2

Use

AAV

Tags

EYFP

Mutation

H134R

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mSptlc2 - 2

Plasmid

#198494

Purpose

lentiviral stable expression of mSptlc2 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mSptlc2 (Sptlc2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-P2A-MCS (Neo)

Plasmid

#89181

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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