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MSGV Rh Acceptor

Plasmid

#64271

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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p3XFlag-YedK

Plasmid

#84567

Purpose

Expresses YedK in mammalian cells

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK

Tags

3xFlag and C-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMM100

Plasmid

#127225

Purpose

AtU6::sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pIA1402

Plasmid

#172403

Purpose

Expression of SARS-COV-2 Nsp12 Y129A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

Y129A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T3_gRNA

Plasmid

#199335

Purpose

pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pIA1408

Plasmid

#172405

Purpose

Expression of SARS-COV-2 Nsp12 D760A in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

Insert

SARS-COV-2 Nsp12 (ORF1ab Synthetic)

Tags

His-SUMO

Expression

Bacterial

Mutation

D760A

Promoter

T7

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pYC12

Plasmid

#63911

Purpose

Initial Overexpressing Vector

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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Paxillin LD

Plasmid

#186150

Purpose

For expressing recombinant paxillin variants in bacteria.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

D5A; D146A; D232A; D267A; D301A

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-Paxillin

Plasmid

#186143

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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FAK

Plasmid

#186141

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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JC101

Plasmid

#104952

Purpose

Methanol induing RFP, estrogen inducing GFP, and IPTG inducing CFP

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

GFP, mCherry, and CFP

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfPar3_3

Plasmid

#26705

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Dog Par3 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Par3 RNAi

Available Since

Jan. 3, 2011

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBC

Plasmid

#69555

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBC (Ubc Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pRAJ31f

Plasmid

#39257

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system (no terminator for the transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTurquoise2-T2A-mCherry

Plasmid

#118442

Purpose

Produces equimolar expression of yeast codon-optimized mTq2 and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2-T2A-mCherry

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ22

Plasmid

#63919

Purpose

Initial replicative vector

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

NATMX

Expression

Yeast

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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Sticky-147-4X

Plasmid

#158575

Purpose

Production of DNA fragment for nucleosome core particle preparation

Depositor

Curt Davey

Article

Sharma et al Nat Commun. 2019 Dec 17;10(1):5751. doi:

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB

Plasmid

#69556

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
JC172

Plasmid

#104948

Purpose

Methanol inducing HSA-hGH fusion protein, and estrogen inducing TEV protease

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

HSA-hGH fusion protein and TEV protease

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymYPET-T2A-mTurquoise2

Plasmid

#118444

Purpose

Produces equimolar expression of ymYPET and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET-T2A-mTurquoise2

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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