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We narrowed to 18,824 results for: nls

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Showing: 4321 - 4340 of 18824 results

  2. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

    Plasmid
    #139990
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 Āµg Transfection-Ready DNA and 100 Āµg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VQR=D1135V/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139990-plasmid-map-sequence-id-275521

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  3. sABE v3.22_N

    Plasmid
    #209553
    Purpose
    pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA
    Depositor
    Xue Gao
    Publication
    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:
    Has service
    50 Āµg Transfection-Ready DNA and 100 Āµg Transfection-Ready DNA
    Insert
    TadA-8e(2-76)-2xFRB
    Expression
    Mammalian
    Promoter
    pCMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209553-plasmid-map-sequence-id-415522

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  4. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid
    #139992
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 Āµg Transfection-Ready DNA and 100 Āµg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139992-plasmid-map-sequence-id-275524

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  5. pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605)

    Plasmid
    #248211
    Purpose
    bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    bpNLS-Stab-exin21-Bxb1(A315R)-Stab
    Use
    CRISPR; In vitro transcription
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Bxb1(A315R)
    Promoter
    CMV and T7
    Available since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248211-plasmid-map-sequence-id-506607

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  7. pPBneo-CIBN-lin-p53CT(98-393)-6KQ-mNeonGreen-NLSx3

    Plasmid
    #241848
    Purpose
    Expresses an improved localizer of Opto-p53 in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.
    Has service
    50 Āµg Transfection-Ready DNA and 100 Āµg Transfection-Ready DNA
    Insert
    p53 (TP53 Human)
    Tags
    CIBN and mNeonGreen
    Expression
    Mammalian
    Mutation
    p53 C-terminus (97-393 aa) containing six acetylaā€¦
    Promoter
    CAGGS promoter
    Available since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    241848-plasmid-map-sequence-id-485119

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  8. pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-5SD-lin-hCRY2

    Plasmid
    #241846
    Purpose
    Expresses an improved actuator of Opto-p53 in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.
    Has service
    50 Āµg Transfection-Ready DNA and 100 Āµg Transfection-Ready DNA
    Insert
    p53 (TP53 Human)
    Tags
    CRY2 and miRFP703
    Expression
    Mammalian
    Mutation
    N-terminus (1-97 aa) containing five phosphorylatā€¦
    Promoter
    CAGGS promoter
    Available since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    241846-plasmid-map-sequence-id-485117

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  11. pAAV_BiPVe4_dTomato_nlsdTomato (AAV PHP.eB)

    Viral prep
    #213936-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiPVe4_dTomato_nlsdTomato (#213936). In addition to the viral particles, you will also receive purified pAAV_BiPVe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the chandelier cell-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Tags
    dTomato
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 4321 - 4340 of 18824 results