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pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

Plasmid

#148402

Purpose

Insect Expression of DmNot1_1674-1962-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1_1674-1962-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMextli-M605D_AB

Plasmid

#148344

Purpose

Insect Expression of DmMextli-M605D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli-M605D (mxt Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R

Plasmid

#147433

Purpose

Insect Expression of DmNot1_1711-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1711-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmMextli_577-620-I583RS590R-GB1_AB

Plasmid

#148357

Purpose

Bacterial Expression of DmMextli_577-620-I583RS590R

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-620-I583RS590R (mxt Fly)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AA

Plasmid

#148272

Purpose

Bacterial Expression of DmThor_50-83-R56ER63E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-R56ER63E (Thor Fly)

Expression

Bacterial

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1148_I

Plasmid

#146604

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Expression

Insect

Mutation

4 silent and two non silent N605S, K759M mutation…

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSmTex-del46-49-V5His6_B

Plasmid

#145995

Purpose

Insect Expression of DmTral-LSmTex-del46-49

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmTex-del46-49 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSmTex-del46-49-V5His6_B

Plasmid

#145985

Purpose

Insect Expression of DmTral-LSmTex-del46-49

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSmTex-del46-49 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D

Plasmid

#146129

Purpose

Insect Expression of DmEDC3_102-330

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_102-330 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmTral-LSm-del46-60-V5His6_C

Plasmid

#146068

Purpose

Insect Expression of DmTral-LSm-del46-60

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm-del46-60 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C

Plasmid

#146046

Purpose

Insect Expression of DmTral-LSm-del46-60

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral-LSm-del46-60 (tral Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG1856_A

Plasmid

#145905

Purpose

Insect Expression of CG1856-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Jan . 15(1):21-32.

Insert

CG1856-3UTR (ttk Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_A

Plasmid

#145894

Purpose

Insect Expression of DmCCR4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmCCR4 (twin Fly)

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

Plasmid

#146957

Purpose

Insect Expression of DmAGO1-K690A

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

DmAGO1-K690A (AGO1 Fly)

Expression

Insect

Mutation

variation with 2 non silent mutations V315A and H…

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-EnR-MCS_N-term

Plasmid

#136884

Purpose

Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-EnR_C-term

Plasmid

#136885

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 TNos:Luc:5'UTR (GB1499)

Plasmid

#160580

Purpose

Reversed sequences of the NOS terminator, Firefly Luciferase CDS and 5' UTR of 35S promoter

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

Luc

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 PhiC31 integrase (GB1561)

Plasmid

#160586

Purpose

Streptomyces phage PhiC31 integrase, adapted for C-terminal fusions

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 linker-RDF (GB2892)

Plasmid

#160604

Purpose

RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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