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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,489 results for: TIL;

Showing: 4341 - 4360 of 4489 results

  2. MLNR-DuET

    Plasmid
    #213339
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MLNR (MLNR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213339-plasmid-map-sequence-id-430913

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  6. LifeAct-14 (LA-14)

    Plasmid
    #158750
    Purpose
    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.
    Depositor
    Minhajuddin Sirajuddin
    Article
    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152
    Insert
    LifeAct-14
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Deletion mutation for last three amino acids from…
    Promoter
    CMV immediate early
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158750-plasmid-map-sequence-id-310555

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  10. pEMI91

    Plasmid
    #74888
    Purpose
    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information
    Depositor
    Piotr Marszalek
    Article
    Scholl et al Biomacromolecules. 2016 Jun 8.
    Insert
    Titin I27 x 9 (TTN Human)
    Tags
    His-tag and Strep-tag II
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74888-plasmid-map-sequence-id-141569

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  11. pFGL1252_TetOFF(Hyg)

    Plasmid
    #118992
    Purpose
    TetOFF (promoter-less) cassette with Hygromycin selection
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    Use
    Fungal expression (in magnaporthe oryzae)
    Mutation
    Codon optimized for Magnaporthe oryzae
    Promoter
    Aspergillus nidulans TrpC promoter, Ustilago mayd…
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    118992-plasmid-map-sequence-id-232118

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  13. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  16. XW17

    Plasmid
    #65835
    Purpose
    Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)
    Depositor
    Kang Shen
    Article
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    OR74A quinic acid utilization activator (qa-1F)
    Tags
    mCherry
    Expression
    Worm
    Mutation
    The endogenous SphI, KpnI and SacI sites in QF ar…
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65835-plasmid-map-sequence-id-120565

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Showing: 4341 - 4360 of 4489 results