We narrowed to 7,548 results for: ski

1. W118-1_hTGM2_C277S-Flag

   Plasmid

   #180412

   Purpose

   lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM2_C277S (TGM2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C277S

   Promoter

   CMV

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK341

   Plasmid

   #192640

   Purpose

   Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpRY
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   SoxS has R93A and S101A mutations and dSpRY has s…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX6P1-HA-MEK1

   Plasmid

   #53159

   Purpose

   Expresses GST-HA tagged human MEK1 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGK-DTAG-NTERM-GFP

   Plasmid

   #185775

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   William Sellers, Alessandra Ianari

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-TAG-NICD

   Plasmid

   #130934

   Purpose

   PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   NICD (NOTCH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR3.1-mGM-CSF

   Plasmid

   #74465

   Purpose

   expression of mGM-CSF in mammalian cells

   Depositor

   Loree Heller

   Article

   Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

   Insert

   granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

   Plasmid

   #138143

   Purpose

   Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiBE3-SpRY-Blast

   Plasmid

   #199303

   Purpose

   Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   FNLS-BE3-SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   2A tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDY1462_SERPINA1_CCA32_Caspase

   Plasmid

   #193194

   Purpose

   Express Caspase conditional upon RNA transcript

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   iCaspase9 (CASP9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLJM1 TMEM192-Flag

    Plasmid

    #112742

    Purpose

    express pLJM1 TMEM192-Flag

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    TMEM192-Flag (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-hSTING (V155M)

    Plasmid

    #214161

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V155M) (STING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V155M

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HiBiT-FZD8

    Plasmid

    #195846

    Purpose

    Expresses human FZD8 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD8 (FZD8 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-CMV-HA-CFP-SLMAP (isoform r)

    Plasmid

    #235684

    Purpose

    Express CFP tagged SLMAP gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    SLMAP (SLMAP Human)
    

    Use

    Lentiviral

    Tags

    HA-CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-CMV-V5-RFP-MST2

    Plasmid

    #235688

    Purpose

    Express RFP tagged MST2 gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    MST2 (STK3 Human)
    

    Use

    Lentiviral

    Tags

    V5-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-hnRNPU-V5

    Plasmid

    #35974

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-HA-mSTING (V154M)

    Plasmid

    #214159

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V154M) (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N153S

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PGK-SMASH-CTERM-GFP

    Plasmid

    #185776

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    William Sellers, Alessandra Ianari

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, SMASh

    Expression

    Mutation

    none

    Promoter

    PGK

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRVdGL-4Cre

    Plasmid

    #98039

    Purpose

    Second-generation rabies viral vector genome

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    mCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRBV5-1

    Plasmid

    #123414

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV5-1 (TRBV5-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_hSyn1_nLightR

    Plasmid

    #187180

    Purpose

    Expresses red norepinephrine indicator nLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Has Service

    AAV9

    Insert

    nLightR
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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