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pJ414 - DANCER-3

Plasmid

#136387

Purpose

Expresses DANCER-3 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-3

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-R1868*-3xFLAG

Plasmid

#130679

Purpose

Expresses human SMCHD1 R1868* with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 R1868* (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

R1868_V2005delinsX

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pPI461

Plasmid

#113215

Purpose

generic knockin vector for C-terminal tagging with mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mCherry

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPI457

Plasmid

#113200

Purpose

shuttle vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, c-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPI418

Plasmid

#113199

Purpose

shuttle vector, N-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, n-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 WT

Plasmid

#87220

Purpose

Encodes Hsh155 WT for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR[traF]

Plasmid

#131615

Purpose

Expresses a functional PuroR only in females and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Insert

PuroR[traF]

Expression

Insect

Promoter

Opie2

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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Vh-HA-3xDHFR

Plasmid

#20219

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-SMCHD1-Exon37-48-3xFLAG

Plasmid

#130688

Purpose

Expresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36-3xFLAG

Plasmid

#130681

Purpose

Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_V2005delinsX

Promoter

CMV

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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2xDHFR-myc

Plasmid

#20215

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xDHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
PSUPER-1660(hPot1)

Plasmid

#11131

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi

Expression

Mammalian

Mutation

N/A

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pPI192

Plasmid

#113219

Purpose

generic knockin vector for C-terminal tagging with mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mCherry

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1019

Plasmid

#108976

Purpose

shuttle vector, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pNP1 AND input A

Plasmid

#107362

Purpose

Input A for pNP1 AND gate sfGFP

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

AND gate input A

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 AND input B

Plasmid

#107363

Purpose

Input B for pNP1 AND gate sfGFP

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

AND gate input B

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1220

Plasmid

#108993

Purpose

integrating expression vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFM111

Plasmid

#40157

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-1N:FLAG (par-1 Nematode)

Tags

Flag and GST

Expression

Bacterial

Mutation

Contains aa 1-964

Promoter

tac

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pET3CTc3A(1-135)

Plasmid

#63138

Purpose

Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

Depositor

Titia Sixma

Article

Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

Insert

Tc3A

Tags

6xHis

Expression

Bacterial

Mutation

1-135 aa bipartite domain

Promoter

T7

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01*(ΔL1+L2)

Plasmid

#80044

Purpose

Expression of EPN-01* mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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