We narrowed to 14,608 results for: SHI

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pet30b-5M SrtA

Plasmid

#51140

Purpose

expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

Depositor

Hidde Ploegh

Article

Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

Insert

S.aureus SrtA 5M

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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Dnase1L3 R206C pIRES-DsRed

Plasmid

#98847

Purpose

Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

Depositor

Peter Keyel

Article

Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

Insert

Dnase1L3 (DNASE1L3 Human)

Tags

IRES DsRed

Expression

Mammalian

Mutation

R206C which greatly reduces nuclease activity

Promoter

CMV

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pUC-fFuCas9-HTBNLS-hph

Plasmid

#121092

Purpose

Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

Depositor

Xiao-Jun Ji

Article

Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

Insert

Cas9 and hygromycin resist gene

Use

CRISPR

Expression

Bacterial

Promoter

gpdA;trpC

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pZS1[FruR-L]

Plasmid

#60742

Purpose

Contains PIq driving expression of the FruR-L, the Fructose inducible chimera with the wt Lac DBD.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

FruR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with FruR L…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP

Plasmid

#190039

Purpose

Expression of FIP200 mutant

Depositor

James Hurley

Article

Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

Insert

FIP200 (RB1CC1 Human)

Tags

GST and MBP

Expression

Mammalian

Mutation

(641-779)deletion & 1363 RDKDLIES 1370 to GSS…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pZS1[GalS-L,LacI-L]

Plasmid

#60759

Purpose

Contains PIq driving expression GalS-L, and PI driving expression of LacI-L.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

GalS-L
wt LacI

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pzs1[GalS-L]

Plasmid

#60743

Purpose

Contains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

GalS-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with GalS L…

Available Since

July 10, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-T]

Plasmid

#60751

Purpose

Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

RbsR-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

Chimeric LacI/GalR repressor with the TAN DBD and…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZS1[RbsR-L]

Plasmid

#60741

Purpose

Contains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

RbsR-L

Use

Synthetic Biology

Expression

Bacterial

Mutation

LacI/GalR repressor chimera. LacI DBD with RbsR L…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pHS9_10xHis_MBP_TEV_dCas9

Plasmid

#244831

Purpose

Bacterial expression of catalytically inactive Cas9 (dCas9) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D10A/H840A

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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