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Showing: 421 - 440 of 505 results

  1. pQE80L TriCatcher-MMP

    Plasmid
    #112632
    Purpose
    Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher
    Depositor
    Mark Howarth
    Article
    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:
    Insert
    TriCatcher-MMP
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    central matrix metalloproteinase-cleavable sequen…
    Promoter
    T5
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112632-plasmid-map-sequence-id-221417

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  2. pAAV-CaMKIIa-ChRger2-TS-EYFP

    Plasmid
    #127238
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger2-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIa
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127238-plasmid-map-sequence-id-247000

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  3. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Use
    Tags
    3x-FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    pTet
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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  5. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  10. pQE80L TriCatcher-RGDSP-MMP

    Plasmid
    #112633
    Purpose
    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher
    Depositor
    Mark Howarth
    Article
    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:
    Insert
    TriCatcher-RGDSP-MMP
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    central matrix metalloproteinase-cleavable sequen…
    Promoter
    T5
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112633-plasmid-map-sequence-id-222151

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  11. pAAV-CaMKIIa-ChRger3-TS-EYFP

    Plasmid
    #127241
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger3-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIa
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127241-plasmid-map-sequence-id-247096

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  12. pAAV-CaMKIIa-ChRger1-TS-EYFP

    Plasmid
    #127244
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CamKIIa
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127244-plasmid-map-sequence-id-247231

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  13. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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Showing: 421 - 440 of 505 results