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pTETDuet_D27NDHFR_R166QTS

Plasmid

#191295

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

D27N DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTETDuet_M42FDHFR_R166QTS

Plasmid

#191296

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_R166QTS

Plasmid

#191297

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

G121V DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YG121VDHFR_R166QTS

Plasmid

#191299

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

F31Y/G121V DHFR, R166Q TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_WTDHFR_R127ATS

Plasmid

#191300

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_D27NDHFR_R127ATS

Plasmid

#191301

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

D27N DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FDHFR_R127ATS

Plasmid

#191302

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_R127ATS

Plasmid

#191303

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

G121V DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YG121VDHFR_R127ATS

Plasmid

#191304

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

F31Y/G121V DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FG121VDHFR_R127ATS

Plasmid

#191305

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F/G121V DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_D27NDHFR_WTTS

Plasmid

#191283

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

D27N DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FDHFR_WTTS

Plasmid

#191284

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_WTTS

Plasmid

#191285

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FG121VDHFR_WTTS

Plasmid

#191286

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F/G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YG121VDHFR_WTTS

Plasmid

#191287

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

F31Y/G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_WTDHFR_Q33STS

Plasmid

#191288

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FDHFR_Q33STS

Plasmid

#191290

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

M42F DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_Q33STS

Plasmid

#191291

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

G121V DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEW8

Plasmid

#84238

Purpose

Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

Depositor

David Weinberg

Article

DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

Insert

HA-mRuby-P2A-eGFP

Tags

HA-mRuby and eGFP

Expression

Yeast

Promoter

GPD

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-pld

Plasmid

#128795

Purpose

expresses E. coli PLD in bacteria

Depositor

Emily Balskus

Article

Chittim et al Nat Microbiol. 2019 Jan;4(1):155-163. do

Insert

Phospholipase D

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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MB 103C ttrSR(m13)-Bxb1_P7-sfGFP_mCherry

Plasmid

#232475

Purpose

Optimized tetrathionate sensor with recombinase switch and fluorescent reporter (GFP), constitutive mCherry

Depositor

Mikhail Shapiro

Article

Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

Inserts

ttrS
ttrR
PttrB185-269
sfGFP
AxeTxe
mCherry
Bxb1 integrase

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL06

Plasmid

#104430

Purpose

super Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

Depositor

Joel Kralj

Article

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

Super Ecliptic pHluorin-mRuby2

Expression

Yeast

Promoter

Tef1

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX304-mNeonGreen

Plasmid

#162034

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Neon Green

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX301-TagRFP

Plasmid

#162035

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

RFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX303-GFP

Plasmid

#162032

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX304-GFP

Plasmid

#162033

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR357.12

Plasmid

#125107

Purpose

Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)134
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Mutation

A84T - see depositor comments below and Q183K-see…

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-GFI1

Plasmid

#16168

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Growth factor independent 1 (GFI1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
Diacylglycerol Kinase Zeta

Plasmid

#85454

Purpose

expression of Diacylglycerol Kinase Zeta

Depositor

Stephen Gee

Article

Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

Insert

Diacylglycerol Kinase Zeta (DGKZ Human)

Tags

3x HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG416GALL-HIV-1_PR

Plasmid

#203493

Purpose

Expresses HIV-1 protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HIV-1 protease

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge