We narrowed to 3,337 results for: GCT

1. pNSU299_GAL2

   Plasmid

   #166088

   Purpose

   Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast

   Depositor

   Rebecca Burgess

   Insert

   GAL2 (GAL2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Tet-inducible

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-TRC.mKO2_shHuR CDS

   Plasmid

   #110426

   Purpose

   TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene and express monomeric Kusabira-Orange2.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 HuR
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA Pol III)

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

   Plasmid

   #177236

   Purpose

   Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase

   Depositor

   Monte Winslow

   Insert

   sgNeo1/sgNT1/sgSnrk
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   bU6/mU6/hU6

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

   Plasmid

   #177234

   Purpose

   Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase

   Depositor

   Monte Winslow

   Insert

   sgNeo1/sgNuak1/sgNuak2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   bU6/mU6/hU6

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

   Plasmid

   #177233

   Purpose

   Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase

   Depositor

   Monte Winslow

   Insert

   sgNeo1/sgPrkaa1/sgPrkaa2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   bU6/mU6/hU6

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_1st

   Plasmid

   #177227

   Purpose

   Expresses Neomycin(bU6), non-targeting (mU6) and Lkb1 (hU6) gRNAs and Cre-recombinase

   Depositor

   Monte Winslow

   Insert

   sgNeo1/sgNT1/sgLkb1_1st
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   bU6/mU6/hU6

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pML107-ARB1

   Plasmid

   #232888

   Purpose

   Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   ARB1 gRNA (ARB1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pML107-YFR054C

   Plasmid

   #232900

   Purpose

   Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   YFR054C gRNA (YFR054C Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pML107-SRP14

   Plasmid

   #232896

   Purpose

   Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   SRP14 gRNA (SRP14 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. bU6-sgLkb1_1st-mU6-sgNeo1-hU6-sgNT

    Plasmid

    #177225

    Purpose

    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Insert

    sgLkb1_1st/sgNeo1/sgNT1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6/mU6/hU6

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. bU6-sgNeo1-mU6-sgLkb1_1st-hU6-sgNT

    Plasmid

    #177226

    Purpose

    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Insert

    sgNeo1/sgLkb1_1st/sgNT1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    bU6/mU6/hU6

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2b:TMV-3NTR

    Plasmid

    #106218

    Purpose

    Provides Nos terminator and 3' part of Tobacco mosaic virus (3NTR, ribozyme) as level 0 GoldenBraid part (GCTT-CGCT)

    Depositor

    Tomáš Moravec

    Insert

    Infectious clone of Tobacco mosaic virus, 3' part
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-PPPR2D-2

    Plasmid

    #179355

    Purpose

    shRNA suppression

    Depositor

    William Hahn

    Article

    Sablina et al Cancer Res. 2010 Dec 15;70(24):10474-84.

    Insert

    B55δ
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Age1

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-puro-hMYC

    Plasmid

    #185376

    Purpose

    For mammalian expression of guide RNA: TGCTGCCAAGAGGGTCAAGT that targets human MYC

    Depositor

    Ayaz Najafov

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZSCAN9.1.0-gDNA

    Plasmid

    #112440

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF193

    Depositor

    Kevin White

    Insert

    ZNF193 (ZNF193 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZBTB40.1.0-gDNA

    Plasmid

    #112431

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZBTB40

    Depositor

    Kevin White

    Insert

    ZBTB40 (ZBTB40 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZNF398.2.0-gDNA

    Plasmid

    #112415

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF398

    Depositor

    Kevin White

    Insert

    ZNF398 (ZNF398 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZNF70.1.0-gDNA

    Plasmid

    #112414

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF70

    Depositor

    Kevin White

    Insert

    ZNF70 (ZNF70 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL60004

    Plasmid

    #117519

    Purpose

    rbcS-E9 terminator Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GCTT:E9t:CGCT:BsaI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge