Addgene

We narrowed to 13,831 results for: NTS

Showing: 421 - 440 of 13831 results

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pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pNP555

Plasmid

#222722

Purpose

sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO225

Plasmid

#235729

Purpose

Protin expression of FL yeast VPS15 -ZZ in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS15

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO290

Plasmid

#235731

Purpose

Protein expression in FL yeast VPS34 D731N mutant in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

Expression

Yeast

Mutation

S2A, D731N

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO478

Plasmid

#235757

Purpose

Expression of FL ScVPS34-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

3xFLAG

Expression

Yeast

Mutation

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO764

Plasmid

#235761

Purpose

Expression of ScVPS38-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO932

Plasmid

#235764

Purpose

Expression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Use

Tags

3xFLAG

Expression

Yeast

Mutation

aa 268-875

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pNTI763 p(Z)-mCherry + UBC9 sgRNA

Plasmid

#164918

Purpose

CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(Z)-mcherry

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

P(Z), synthetic promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

Plasmid

#164916

Purpose

Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

GCN4(UTR)-ZEM(TF)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

PGK1 (yeast)

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

Plasmid

#115432

Purpose

Vector for expressing RPL28 with mutagenized exon2

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Use

Tags

Expression

Yeast

Mutation

exon 2 deleted, with MfeI / NheI cloning site

Promoter

Ashbya gospii TEF1 and endogenous

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pNP1723

Plasmid

#222727

Purpose

sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pICH41331::pU6cm-pegR::EURb7

Plasmid

#227695

Purpose

Golden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

Altered epegRNA backbone with cloning sites for gRNA and RTT.

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

U6 composite

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pICH47742:: pRPS5a-PE2max-NC::tHSP

Plasmid

#227694

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by pRPS5a and terminated by tHSP

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pRPS5a

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pICH47742::p35S-PE2max-NC::tEURb7

Plasmid

#227693

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI821

Plasmid

#216630

Purpose

Tet-inducible Cal-ID (V5) for Sleeping Beauty transposon system

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

Sleeping beauty transposon system

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI822

Plasmid

#216631

Purpose

Tet-inducible cpTurboID (V5) for Sleeping Beauty transposon system

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

Sleeping beauty transposon system

Tags

V5

Expression

Mammalian

Mutation

L120P

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI934

Plasmid

#216637

Purpose

Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNP568

Plasmid

#222725

Purpose

pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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