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Plasmid#190380PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XRpaRO-min35S::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XRpaRO-min35S::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2485
Plasmid#190371PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-McbRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-McbRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2463
Plasmid#190370PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-QacRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-QacRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2275
Plasmid#190361PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2224
Plasmid#190358PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::GFPint-ADHterm)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::GFPint-ADHterm
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2193
Plasmid#190357PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XTetRO-min35S::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XTetRO-min35S::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2178
Plasmid#190351PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XIcaRO-min35S::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XIcaRO-min35S::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2177
Plasmid#190350PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-min35S::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-min35S::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Spec(x2)-DocI
Plasmid#58713PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertSpec(x2)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mNeonGreen
Plasmid#175508PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16
Plasmid#172746PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-16
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-4GS-LaG-2
Plasmid#172764PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)DepositorInsertLaG-16-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-30
Plasmid#172754PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-30
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-3
Plasmid#172769PurposeBacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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PB-CT3G-CERP2-MG-IRES2-mCherry
Plasmid#175507PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only