We narrowed to 2,345 results for: PUC19

1. pGGE_RT_Sa_sgRNA

   Plasmid

   #136978

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-NEO-GFP-RFP

   Plasmid

   #157952

   Purpose

   seamless gene editing and component of Piggybac transposase system

   Depositor

   Yan-zhen Bi

   Type

   Empty backbone

   Use

   Mouse Targeting; Gene targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB-Bleo STAT3

   Plasmid

   #139184

   Purpose

   Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.

   Depositor

   Neal Devaraj

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   STAT3

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS201-ophi

   Plasmid

   #227349

   Purpose

   Heterologous production of ophiobolin C in Aspergillus oryzae

   Depositor

   Jun-ichi Maruyama

   Insert

   The hoomlogous regions of the HS201 locus and expression cassettes of the AcOS, oblDBm, and oblBBm genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pwAB-erg8erg19idi1erg20

   Plasmid

   #227345

   Purpose

   Overexpression of the erg8, erg19, idi1, and erg20 genes in Aspergillus oryzae

   Depositor

   Jun-ichi Maruyama

   Insert

   Expression cassettes of the erg8, erg19, idi1, and erg20 genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdadhDN-tefAO217

   Plasmid

   #227341

   Purpose

   Replacement of the adh gene with the expression cassette of the AO217 gene

   Depositor

   Jun-ichi Maruyama

   Insert

   Flanking regions of adh (AO090009000634) and expression cassette of AO217 (AO090120000217)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. ppG-ple12

   Plasmid

   #227339

   Purpose

   Heterologous production of pleuromutilin in Aspergillus oryzae

   Depositor

   Jun-ichi Maruyama

   Insert

   The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pwA-ple34

   Plasmid

   #227337

   Purpose

   Heterologous production of pleuromutilin in Aspergillus oryzae

   Depositor

   Jun-ichi Maruyama

   Insert

   The upstream/inside regions of the wA gene and expression cassettes of the ple3 and ple4 genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

   Plasmid

   #155306

   Purpose

   Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTOX-donor

    Plasmid

    #132516

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TOX (TOX Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTBX18-donor

    Plasmid

    #176370

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    TBX18 (TBX18 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZNF468-donor

    Plasmid

    #176350

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    ZNF468 (ZNF468 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-dirTopo-P2A-aGFP

    Plasmid

    #61974

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC-dirTopo-AU1-aGFP

    Plasmid

    #61973

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Promoter

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIAeBlue

    Plasmid

    #185891

    Purpose

    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAW8-aid-Turg1:kanMX6

    Plasmid

    #169354

    Purpose

    C-terminal aid degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOTX2-donor

    Plasmid

    #176358

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    OTX2 (OTX2 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIEforRed

    Plasmid

    #185892

    Purpose

    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSTAT5B-donor

    Plasmid

    #176339

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    STAT5B (STAT5B Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMDW133

    Plasmid

    #227248

    Purpose

    Lambda DNA for torsional templates

    Depositor

    Michelle Wang

    Insert

    Lambda DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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