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Addgene
Showing: 421 - 440 of 2310 results
  1. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pIAeBlue

    Plasmid
    #185891
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus
    Depositor
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pAW8-aid-Turg1:kanMX6

    Plasmid
    #169354
    Purpose
    C-terminal aid degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pOTX2-donor

    Plasmid
    #176358
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    OTX2 (OTX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pIEforRed

    Plasmid
    #185892
    Purpose
    In vivo gene amplification (HamAmp) of EforRed expression module at RPL25 locus
    Depositor
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pSTAT5B-donor

    Plasmid
    #176339
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    STAT5B (STAT5B Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pMDW133

    Plasmid
    #227248
    Purpose
    Lambda DNA for torsional templates
    Depositor
    Insert
    Lambda DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pXylP-HygR

    Plasmid
    #168131
    Purpose
    Expression of HygR from the xylose-inducible xylP promoter
    Depositor
    Insert
    XylP-HygR
    Use
    Aspergillus expression
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    xylP
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pOSR1-donor

    Plasmid
    #176363
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    OSR1 (OSR1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pZNF627-donor

    Plasmid
    #176365
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    ZNF627 (ZNF627 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pZNF79-donor

    Plasmid
    #176371
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    ZNF79 (ZNF79 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pSNAPC5-donor

    Plasmid
    #176341
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    SNAPC5 (SNAPC5 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pZNF449-donor

    Plasmid
    #132515
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Insert
    ZNF449 (ZNF449 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  15. pNPAS1-donor

    Plasmid
    #176346
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    NPAS1 (NPAS1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pSOX15-donor

    Plasmid
    #176356
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    SOX15 (SOX15 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pAW8-aid-eDSR-Turg1:kanMX6

    Plasmid
    #169362
    Purpose
    C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pUC-ODN2

    Plasmid
    #208643
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).
    Depositor
    Type
    Empty backbone
    Use
    Long single strand dna production
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. pPRDM15-donor

    Plasmid
    #176373
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    PRDM15 (PRDM15 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pZIC3-donor

    Plasmid
    #176361
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    ZIC3 (ZIC3 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 421 - 440 of 2310 results