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pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

Plasmid

#138985

Purpose

IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

Plasmid

#138984

Purpose

IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pPB12A

Plasmid

#210035

Purpose

Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas12a
rtta-T2A-puro

Use

CRISPR

Tags

nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

Plasmid

#130275

Purpose

Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

Arch(D95N)

Tags

2x Myc-tag

Expression

Worm

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pNH13 (pmyo-2::QuasAr::mOrange)

Plasmid

#130273

Purpose

Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

QuasAr::mOrange

Tags

mOrange

Expression

Worm

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168170

Purpose

Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pNH12 (pmyo-2::MacQ::mCitrine)

Plasmid

#130274

Purpose

Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

MacQ::mCitrine

Tags

mCitrine

Expression

Worm

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-meGFP-Myo6

Plasmid

#225227

Purpose

Expresses GFP-tagged Myo6

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Myo6 (MYO6 )

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-CAG-meGFP-Myo6

Plasmid

#219575

Purpose

Lentiviral construct expressing GFP tagged human Myo6

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Myo6 (MYO6 )

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pGuide-RUNX3

Plasmid

#246659

Purpose

Guide RNA for human RUNX3

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

RUNX3 guide RNA (RUNX3 Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPB9

Plasmid

#210036

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
puro-T2A-eGFP

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]

Plasmid

#198381

Purpose

Expression of SYPB::CRY2olig(535) in neurons of Danio rerio

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

UAS:sypb-egfp-cry2

Use

Tol2

Tags

EGFP

Mutation

D387A

Promoter

UAS

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 Del AA 6

Plasmid

#184024

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 689 to 694.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8 Del AA 6 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of amino acids 689 to 694

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-1

Plasmid

#184013

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 685-706.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-1 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of amino acids 685-706.

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#117429

Purpose

Expression of ARL1

Depositor

Richard Kahn

Article

Van Valkenburgh et al J Biol Chem. 2001 Jun 22;276(25):22826-3

Insert

ARL1 (ARL1 Human)

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR

Plasmid

#171240

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor (AR Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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7M8

Plasmid

#64839

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

Insert

7M8 cap

Use

AAV

Mutation

7mer insertion in the AAV2 cap

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA flag-Msi1

Plasmid

#184029

Purpose

Msi1 clone with N-terminal flag tag

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Msi1 (Msi1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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HA-HA-BI-AR

Plasmid

#171234

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor (hAR)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor (AR Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pPB9P2

Plasmid

#210030

Purpose

Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

prime editor 2
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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