We narrowed to 675 results for: intein
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Plasmid#241012PurposeVidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.DepositorInsertGSS-6xHis-SUMO-VidC-bla-FLAG
TagsFLAG-tag, His-tag, and SUMO-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG
Plasmid#241011PurposeVidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.DepositorInsertGSS-6xHis-SUMO-VidC-EGF-FLAG
TagsFLAG-tag, His-tag, and SUMO-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp61(I342G)
Plasmid#228208PurposeExpression in BL21(DE3) of T4 primase I342GDepositorInsert61 (61 Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialMutationMutated isoleucine 342 to glycine (I342G), improv…PromoterT7Available SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
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IBMc397
Plasmid#161955PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHHDepositorInsertpSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
ExpressionBacterialPromoterPBADAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc180
Plasmid#161942PurposeCarries Golden Gate substitution insert for acVHH-assisted bisection mappingDepositorInsertpSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
ExpressionBacterialPromoterPBADAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc367
Plasmid#161947PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20DepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
ExpressionBacterialAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc228
Plasmid#161946PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetRDepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
ExpressionBacterialAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc226
Plasmid#161944PurposeContains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmidDepositorInsertpSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
ExpressionBacterialPromoterPBADAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ATG-3xHA-S11
Plasmid#108238PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plantsDepositorInsertATG-3xHA-S11
UseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC-hXRCC1-K176R-IntE2
Plasmid#52277PurposeBacterial expression of human XRCC1-K176R C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertXRCC1 (XRCC1 Human)
TagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK176R, SUMO acceptor site mutantPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-mTDG-K341R-IntE2
Plasmid#52269PurposeBacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertTDG (Tdg Mouse)
TagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK341R, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TnY
Plasmid#163975PurposeIPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.DepositorInsertTnY
TagsMxe intein - Chitin-binding domainExpressionBacterialAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-tTAc
Plasmid#172123PurposeConstitutive expression of InteinC-tTACDepositorInsertInteinC-tTAc
UseAAVPromoterEF1aAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLAC
Plasmid#45072Purposelacritin-intein fusion plasmid coding for intact mature lacritin without signal peptideDepositorInsertLacritin (LACRT Human)
UseProtein expression vectorTagsInteinExpressionBacterialPromoterT7Available SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCaMKII-tTAc
Plasmid#172125PurposeExpression of InteinC-tTAC in CaMKII positive cellsDepositorInsertpCaMKII, tTAc
UseAAVPromoterCaMKIIAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-H2B(1-114)_wt
Plasmid#118252PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNaDepositorInsertxlH2B(1-114)
TagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialPromoterT7Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only