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pDRF1-GW ymTurquoise2-T2A-mCherry

Plasmid

#118442

Purpose

Produces equimolar expression of yeast codon-optimized mTq2 and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2-T2A-mCherry

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Sticky-147-4X

Plasmid

#158575

Purpose

Production of DNA fragment for nucleosome core particle preparation

Depositor

Curt Davey

Article

Sharma et al Nat Commun. 2019 Dec 17;10(1):5751. doi:

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB

Plasmid

#69556

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
JC172

Plasmid

#104948

Purpose

Methanol inducing HSA-hGH fusion protein, and estrogen inducing TEV protease

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

HSA-hGH fusion protein and TEV protease

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymYPET-T2A-mTurquoise2

Plasmid

#118444

Purpose

Produces equimolar expression of ymYPET and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET-T2A-mTurquoise2

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ytdtomato-T2A-mTurquoise2

Plasmid

#118451

Purpose

Produces equimolar expression of yeast codon-optimized tdTomato and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdtomato-T2A-mTurquoise2

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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JC031

Plasmid

#104953

Purpose

Methanol inducing hGH, estrogen inducing G-CSF, and IPTG inducing Interferon

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

hGH, Interferon and G-CSF

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_SSF_MOR_WT_APEX2

Plasmid

#250773

Purpose

Mammalian cell expression of the mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(WT)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Promoter

UBC

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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puDNA5_SSF_MOR_WT_APEX2

Plasmid

#250759

Purpose

Mammalian cell expression of the wildtype mu opioid receptor

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

MOR(WT)

Tags

APEX2 and Signal Sequence FLAG

Expression

Mammalian

Promoter

UBC

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5_TO_FRT_myc_GLUT4_WT_APEX2

Plasmid

#250766

Purpose

Mammalian cell expression of GLUT4

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

GLUT4(WT)

Tags

APEX2 and myc (in exofacial loop)

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAM198

Plasmid

#227657

Purpose

pRS305 His-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

His-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM200

Plasmid

#227659

Purpose

pRS305 3XFLAG-HRV3C-Rpn5 WT

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3X FLAG-HRV3C-

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM202

Plasmid

#227661

Purpose

pRS316 Rpn6 promoter-Rpn5 ORF-Rpn5 terminator

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Expression

Yeast

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET30a- phage MazG-like/Atd1

Plasmid

#195244

Purpose

Expersses phage MazG-like/Atd1

Depositor

Douglas Feldman

Article

Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

Insert

phage MazG

Tags

Flag and TwinStrep

Expression

Bacterial

Mutation

Catalytic site mutant

Promoter

T7

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T2_gRNA

Plasmid

#197647

Purpose

pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type II sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T1_gRNA

Plasmid

#199332

Purpose

pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type I sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRS304-PTDH3-HEXAMER

Plasmid

#190251

Purpose

Expression hexamer component in yeast

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

hexamer

Tags

mCherry

Expression

Yeast

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Paxillin DN

Plasmid

#186151

Purpose

For expressing recombinant paxillin variants in bacteria.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxilln residues 1-321 added to N-terminus of WT …

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMC_cherry donor_Rosa target (pE002)

Plasmid

#149348

Purpose

Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMC_cherry donor_Polb target (pE038)

Plasmid

#149349

Purpose

Replace donor vector for cherry insertion using the human Polb guide RNA

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

cherry

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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