We narrowed to 6,247 results for: nls

1. pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

   Plasmid

   #227012

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Promoter

   UbC

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MCP/PCP red RNA Lantern

   Plasmid

   #226097

   Purpose

   RNA tracking

   Depositor

   Jennifer Prescher

   Article

   Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

   Insert

   NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-43 mRuby K84ONBK

   Plasmid

   #216226

   Purpose

   TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   Transactive response DNA binding protein of 43 kDa (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Lysine 84 mutated to a TAG stop codon

   Promoter

   CMV Promoter

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP782_Traptavidin-KRAB-CO

   Plasmid

   #211775

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-DNMT3A
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available Since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLP785_Traptavidin-DNMT3A-CO

   Plasmid

   #211778

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-EZH2
   

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP781_SnoopCatcher-KRAB-CO

   Plasmid

   #211774

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-KRAB
   

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pROF018

   Plasmid

   #155325

   Purpose

   Constitutive expression of KRAB-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-NLS-KRAB-EL222-Tnos
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pROF050

   Plasmid

   #155327

   Purpose

   Constitutive expression of BRD-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-BRD-NLS-EL222-Tnos
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB(EXPR-PYL-HDAC5-NEO)

   Plasmid

   #114395

   Purpose

   For PYL-HDAC5 expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5
   

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   D593E compared with NCBI reference NP_005465.2

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB-HDAC5(mut-H-A)

    Plasmid

    #114397

    Purpose

    For PYL-HDAC5 H1006A expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 H1006A
    

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    D593E compared to NCBI ref NP_005465.2

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAB-EXPR(PYL-cat_dom_HDAC5)

    Plasmid

    #114401

    Purpose

    For PYL-HDAC5 catalytic domain expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 catalytic domain
    

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    S220F and the deletion of M221

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAB-EXPR(PYL-Nterm_dom_HDAC5)

    Plasmid

    #114402

    Purpose

    For PYL-HDAC5 N terminal domain expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 N terminal domain
    

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    D593E compared to NCBI ref NP_005465.2

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ265K

    Plasmid

    #173997

    Purpose

    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    UNG-rAPOBEC1(R33A)-zCas9(D10A)
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    R33A in rAPOBEC1 and D10A in zCas9

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PlmdCas12e VPR

    Plasmid

    #188516

    Purpose

    Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-FLAG-VPR

    Expression

    Mammalian

    Mutation

    D659A/E756A/D922A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. KBB438

    Plasmid

    #185092

    Purpose

    GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Expression

    Yeast

    Mutation

    Rfa1 with amino acids 338-343 deleted

    Promoter

    GAL1

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. L2_NOP1gRNA-Cas9-CsA

    Plasmid

    #136139

    Purpose

    Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT174_GalL_A3A-Y130F-BE3

    Plasmid

    #145123

    Purpose

    Expressing base editor A3A-Y130F-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(Y130F)-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(Y130F); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT173_GalL_A3A-R128A-BE3

    Plasmid

    #145122

    Purpose

    Expressing base editor A3A-R128A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(R128A)-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    A3A(R128A); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJT113_GalL_cCDA1-xBE3

    Plasmid

    #145079

    Purpose

    Expressing base editor cCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-xBE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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