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pEGFP-C1 Slp2-a E11A/R32A

Plasmid

#40055

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a E11A/R32A (Sytl2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Glutamate 11 to Alanine, Arginine 32 to Alanine

Promoter

CMV promoter

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
integrin alpha9 / alpha4 pcDNA1 neo

Plasmid

#13587

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 / alpha 4 (ITGA9 Human)

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 / alpha2 pcDNA1 neo

Plasmid

#13586

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 / alpha 2 (ITGA9 Human)

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Btsz2

Plasmid

#40068

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Btsz2 (btsz Fly)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
ePB-Puro_TT-hYAP-5SA-P2A-tdTomato-CAAX

Plasmid

#252251

Purpose

A PiggyBac donor vector for Dox-inducible expression of YAP-5SA and membrane-localized tdTomato.

Depositor

Jun Wu

Article

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

YAP-5SA (YAP1 Human)

Use

Piggybac transposon donor vector

Tags

P2A-tdTomato-CAAX

Expression

Mammalian

Promoter

tight TRE

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET14-H2Kb-ST3

Plasmid

#223221

Purpose

Expression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteria

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Expression of soluble mouse MHC H2KB heavy chain (HLA-A Mouse)

Tags

Spytag

Expression

Bacterial

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-TAF5-FL

Plasmid

#209051

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to full length TAF5 isoform

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-EGFP

Plasmid

#209058

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged EGFP

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

EGFP

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-TAF5-FL

Plasmid

#209049

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-749_HA-GD2-28z_CAR_TFAP4

Plasmid

#207490

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-852_HA-GD2-28z_TFAP4-HA

Plasmid

#207502

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-858_CD19-BBz_CAR_TFAP4

Plasmid

#207503

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-BBz_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

Plasmid

#204214

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of DNA binding domain (delDBD)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

Plasmid

#204191

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of R162

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

Plasmid

#204213

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on I207M

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204211

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on Q175* (*termination)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204210

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Point mutation on R178* (*termination)

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

Plasmid

#204212

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NK2 homeobox 1 (NKX2-1 Human)

Use

Lentiviral

Tags

Point mutation on I207F

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRi

Plasmid

#134989

Purpose

dCas9-mediated inactivation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo E55A E57A K58A nsp1

Plasmid

#175427

Purpose

Expresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nsp1 E55A E57A K58A

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

N-terminal mutants E55A E57A K58A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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