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pGAD-Atg17(L313D I314K)

Plasmid

#121390

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 313 to Aspartic acid; Isoleucine 314 to L…

Promoter

ADH1 promoter

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-Atg17(416) (L105D)

Plasmid

#121405

Purpose

Expressing Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-ATG17 ORF-CYC1 terminator

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

CUP1 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shLARP7

Plasmid

#113537

Purpose

Retroviral vector designed to knock down LARP7.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shLARP7 (LARP7 Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa432-689

Plasmid

#113543

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa400-689

Plasmid

#113542

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa1-400

Plasmid

#113541

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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KG#493

Plasmid

#110932

Purpose

Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

Depositor

Kenneth Miller

Article

Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

Inserts

unc-17 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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KG#94

Plasmid

#110931

Purpose

Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

Depositor

Kenneth Miller

Article

Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

Inserts

unc-17 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-CenpB 1-166

Plasmid

#108474

Purpose

expression of vsv-CenpB 1-166

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB 1-166 AA (CENPB Human)

Tags

VSV

Expression

Mammalian

Mutation

encodes only the DNA-binding domain of CENP-B

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Mis12-dCEN-Incenp

Plasmid

#108477

Purpose

expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-vsv-Mis12-dCEN-Incenp-EGFP

Plasmid

#108478

Purpose

expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_3xFLAG

Plasmid

#101653

Purpose

Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, 3xFLAG tag

Tags

3xFLAG tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom2.5

Plasmid

#87351

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

2.5 kbp proximal promoter of human MCT2 (nested d…

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom1.1

Plasmid

#87352

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

1.1 kbp proximal promoter of human MCT2 (nested d…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom3.2

Plasmid

#87349

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

3.2 kbp proximal promoter of MCT2 (nested deletio…

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX335_HR_Prnp_3

Plasmid

#78622

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_Prnp_SAM2

Plasmid

#78625

Purpose

Encodes sgRNA2.0 targeting 138nt upstream of TSS

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_Prnp_SAM1

Plasmid

#78624

Purpose

Encodes sgRNA2.0 targeting 158nt upstream of TSS

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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