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pBEL1727

Plasmid

#139918

Purpose

Expression plasmid for LetB mutant Y814Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; Y814Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1726

Plasmid

#139917

Purpose

Expression plasmid for LetB mutant W476pa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; W476Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1725

Plasmid

#139916

Purpose

Expression plasmid for LetB mutant F468Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; F468Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1811

Plasmid

#139915

Purpose

Expression plasmid for LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L359N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1810

Plasmid

#139914

Purpose

Expression plasmid for LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L358N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1809

Plasmid

#139913

Purpose

Expression plasmid for LetB mutant L355N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L355N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1808

Plasmid

#139912

Purpose

Expression plasmid for LetB mutant V247N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; V247N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1807

Plasmid

#139911

Purpose

Expression plasmid for LetB mutant L246N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L246N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1806

Plasmid

#139910

Purpose

Expression plasmid for LetB mutant L243N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; L243N)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1862

Plasmid

#139909

Purpose

Complementation plasmid for LetAB with LetB mutant L359N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L359N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1861

Plasmid

#139908

Purpose

Complementation plasmid for LetAB with LetB mutant L358N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L358N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1860

Plasmid

#139907

Purpose

Complementation plasmid for LetAB with LetB mutant L355N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L355N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1857

Plasmid

#139904

Purpose

Complementation plasmid for LetAB with LetB mutant L243N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L243N)

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1584

Plasmid

#139875

Purpose

Expression plasmid of rings 2 and 3 of LetB for crystallography

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(159-383)-6xHis

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAW228

Plasmid

#113242

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->tAggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW227

Plasmid

#113241

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

TTGGTAG->Atggtag

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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relAE335Q_NS1

Plasmid

#100917

Purpose

expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli with point mutation E335Q

Use

Cyanobacteria cloning vector

Tags

FLAG

Mutation

E 335 converted to Q

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB325

Plasmid

#113669

Purpose

expresses Ulp1_R1 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R1

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAW207

Plasmid

#113247

Purpose

E. coli expression vector for IHFa K20A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa K20A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

K20A

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAW205

Plasmid

#113245

Purpose

E. coli expression vector for IHFa D14A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa D14A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

D14A

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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