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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,956 results for: codon optimized

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Showing: 4401 - 4420 of 4956 results

  1. HiFi iFE

    Plasmid
    #190141
    Purpose
    Expresses HiFiCas9-POLB (HiFi iFE)
    Depositor
    Timothy Lu
    Publication
    Nakade et al bioRxiv 2022.12.05.518807
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HiFi Cas9-POLB
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    SpCas9 R691A mutant, Codon Optimized for mammalia…
    Promoter
    CBh
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190141-plasmid-map-sequence-id-377886

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  2. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162678-plasmid-map-sequence-id-321524

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  3. PECR

    Plasmid
    #39158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PECR (PECR Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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  8. pJW1594

    Plasmid
    #121061
    Purpose
    TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    TagRFP-T^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121061-plasmid-map-sequence-id-235980

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  12. HSD17B14

    Plasmid
    #39160
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B14 (HSD17B14 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pPF4213

    Plasmid
    #254224
    Purpose
    Mutagenesis plasmid encoding tnpA and the tn5 transposon harbouring AcrVIA1
    Depositor
    Peter Fineran
    Insert
    AcrVIA1
    Use
    Transposon insertion mutagenesis vector
    Mutation
    Codon optimized for E. coli.
    Promoter
    js23100
    Available since
    July 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    254224-plasmid-map-sequence-id-517553

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  15. pCAG-memRFP-3xPax7gRNA

    Plasmid
    #224569
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Publication
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Mutation
    Codon optimized
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224569-plasmid-map-sequence-id-451497

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Showing: 4401 - 4420 of 4956 results