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We narrowed to 8,985 results for: codon optimized

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Showing: 4401 - 4420 of 8985 results

  8. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  9. pBT3000

    Plasmid
    #122536
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::tram-1 (tram-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122536-plasmid-map-sequence-id-238187

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  10. pSL8215 (dCas12f–σE (sigmaE) system, entry vector)

    Plasmid
    #254272
    Purpose
    Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with BsaI-containing restriction sites for guide RNA cloning
    Depositor
    Samuel H. Sternberg
    Article
    Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-
    Inserts
    dCas12f
    sigma E
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23105
    Available Since
    April 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    254272-plasmid-map-sequence-id-505316

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  13. SpRY

    Plasmid
    #198544
    Purpose
    Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198544-plasmid-map-sequence-id-390296

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  18. P25-pZE-SUMO-PbTTA

    Plasmid
    #204634
    Purpose
    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
    Depositor
    Aditya Kunjapur
    Article
    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:
    Insert
    His-SUMO-TEV-PbTTA
    Use
    Synthetic Biology
    Tags
    His Tag; SUMO Tag; TEV Protease Site
    Expression
    Bacterial
    Promoter
    PLtetO-1 promoter
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204634-plasmid-map-sequence-id-414414

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  19. pJS145

    Plasmid
    #179159
    Purpose
    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
    Depositor
    Iva Greenwald
    Article
    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11
    Insert
    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    Expression
    Worm
    Mutation
    Flexon replacing 2nd intron of 2xNLS::GFP
    Promoter
    rps-27p
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    179159-plasmid-map-sequence-id-352801

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  20. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid
    #160437
    Purpose
    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
    Depositor
    Nicholas Tolwinski
    Article
    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi
    Insert
    Homo sapiens amyloid beta precursor protein (APP Nematode, Human)
    Tags
    Cleavage signal
    Expression
    Bacterial
    Mutation
    Codon-optimized for expression in C. elegans
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    160437-plasmid-map-sequence-id-314980

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Showing: 4401 - 4420 of 8985 results