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AAVS1 intron 1 gRNA 27

Plasmid

#132396

Purpose

Targets AAVS1 intron 1, gRNA: ACCCCACAGTGGGGCCACTA, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pECE beta1 R760A

Plasmid

#50511

Depositor

Kenneth Yamada

Article

Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

Insert

integrin beta1 (ITGB1 Human)

Expression

Mammalian

Mutation

R760A

Promoter

SV40

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
VN1287 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235116

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1262 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235117

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1852 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235118

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses ASf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN550 pAC-cI_E34P-Asf1A_Term_f1-pOL2-62_L-eGFP-KanR

Plasmid

#235102

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

Asf1A (ASF1A Human)

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN991 pB2Hws_v9_cI-Asf1B+rpoA-IP1

Plasmid

#235105

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1043 pB2Hws_v9_cI-Asf1B+rpoA-IP2

Plasmid

#235106

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN990 pB2Hws_v9_cI-Asf1B+rpoA-IP3

Plasmid

#235107

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1335 pB2Hws_v9_cI-Asf1B+rpoA-IP4

Plasmid

#235108

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1312 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_eGFP-wcKanR

Plasmid

#235109

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3mut3A

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1313 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_eGFP-wcKanR

Plasmid

#235110

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1314 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_eGFP-wcKanR

Plasmid

#235111

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1315 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_eGFP-wcKanR

Plasmid

#235112

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1316 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP4_Ddcm_eGFP-wcKanR

Plasmid

#235113

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1264 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235114

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3mut3A

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHCFnc/V5

Plasmid

#247003

Purpose

Expresses non cleavable HCF-1 with carboxy terminal V5 tag under control of the CMV promoter

Depositor

Thomas Kristie

Article

Vogel et al Proc Natl Acad Sci U S A. 2006 May 2;103

Insert

HCF-1 (HCFC1 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

Glu to Ala change at amino acid positions 1019,10…

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMBS-864

Plasmid

#218018

Purpose

GST-tag fused to TEV Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

GST-tag

Use

Synthetic Biology

Tags

TEV protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-848

Plasmid

#218003

Purpose

GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

GST-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-849

Plasmid

#218004

Purpose

8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge