We narrowed to 14,582 results for: eng
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Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
ExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miRE_shBRD9_508-SV40-GFP
Plasmid#75138PurposeRetroviral expression plasmid encoding shBRD9_508 (targeting human BRD)DepositorInsertshBRD9_508
UseRetroviralAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGE001
Plasmid#48839PurposeProvides the rbcS terminator as GreenGate module.DepositorInsertrbcS terminator
UseGolden gate compatible cloning vectorAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST26-PPP2CB-C-HA
Plasmid#195179PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
placZα-tdMCP
Plasmid#191132PurposeExpression of tdMCP from a lacZα inducible promoterDepositorInsertdMCP_1
UseSynthetic BiologyPromoterlac-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hGFAP-Pink Flamindo-WPRE-SV40
Plasmid#178790PurposeAAV vector to express the red cAMP indicator in astrocytesDepositorPublicationInsertPink Flamindo
UseAAVPromoterhGFAPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2359 - intron GG2 - 250bp
Plasmid#159878PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG2 - 250bp
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2515b
Plasmid#91073PurposeModule B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol IIIDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRPromoterAtU6Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mPapaya1.0
Plasmid#52304PurposeAn engineered monomeric Zoanthus sp. yellow fluorescent protein called mpapayaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfluorescent protein mpapaya
ExpressionMammalianAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV_CAG_Gag-MCP
Plasmid#212637PurposeLentivirus vector to express an MLV Gag-MCP fusion proteinDepositorInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UseLentiviralTagsMS2 Capsid Protein (N55K)ExpressionMammalianPromoterCAGAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-T7-NlaCas3-NLS-6xHis
Plasmid#180214PurposeExpressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.ColiDepositorInsertNlaCas3c
Tags6xHis and NLSExpressionBacterialPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBad-UGAcS
Plasmid#172137Purposebacteria expression plasmid for a UDP-GlcNAc sensorDepositorInsertUGAcS
ExpressionBacterialMutationH605Q- please see depositor commentsPromoteraraBADAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA
Plasmid#52549Purposetransgenic construct for expression of GFP and FlpoDepositorInsertsPhiC31o attB (full length)
GFP
Flpo recombinase
ExpressionMammalianAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLAC
Plasmid#45072Purposelacritin-intein fusion plasmid coding for intact mature lacritin without signal peptideDepositorInsertLacritin (LACRT Human)
UseProtein expression vectorTagsInteinExpressionBacterialPromoterT7Available sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDIV151
Plasmid#226180PurposeExpresses Cas9 in a backbone compatible with K. phaffii.DepositorInsertCas9
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterGAP1Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-AcHELIX-KanR (CJT96)
Plasmid#181794PurposepDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-STChRger2-TS-EYFP
Plasmid#129393PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianPromoterCaMKIIaAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-H-Luc_nucl
Plasmid#113931PurposePlasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH-Luc_nucl
UseLuciferaseExpressionMammalianPromoterCMVAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-N3FLAG-MYB-FL
Plasmid#105609Purposetransient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cellsDepositorPublicationAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSuper.Retro.Puro-WDR5 shRNA 2
Plasmid#59975PurposeKnockdown of WDR5DepositorInsertWDR5 shRNA
UseRetroviralAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only