We narrowed to 19,702 results for: ACE;

1. pX458-PLK4

   Plasmid

   #227310

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of PLK4 (PLK4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCD-cTEVP-RBD

   Plasmid

   #226233

   Purpose

   Express cTEVP-RBD fusion

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   cTEVP-RBD (TEVgp1 Tobacco etch virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCD-nTEVP-RBD

   Plasmid

   #226232

   Purpose

   Express nTEVP-RBD fusion

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   nTEVP-RBD (TEVgp1 Tobacco etch virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCD-HCVP

   Plasmid

   #226227

   Purpose

   Express HCVP

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   HCVP (POLY Hepatitis C virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-C-mScarlet-EFS-Zeo

   Plasmid

   #227261

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Golgi-deltaCKAR

   Plasmid

   #222428

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Golgi delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUt-MONARCH 2.0_crEGFP

   Plasmid

   #224793

   Purpose

   LPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Inserts

   crEGFP
   Triplex stablier
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-d2i

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC-hU6-2O-crScaffold_SV40-BFP

   Plasmid

   #224861

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6-2xTetO

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC-hU6-2O-crScaffold_EF1a-BFP

   Plasmid

   #224862

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6-2xTetO

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACYCDuet-1:ChrA-C63S

    Plasmid

    #225080

    Purpose

    Expresses precursor peptide ChrA-C63S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C63S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C63S

    Promoter

    T7

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a:ChrH

    Plasmid

    #225084

    Purpose

    Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrH
    

    Use

    Tags

    His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a:ChrI

    Plasmid

    #225085

    Purpose

    Expresses anthrone-oxygenase like enzyme ChrI in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrI
    

    Use

    Tags

    His6 and His6-Thrombin

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225062

    Purpose

    Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225063

    Purpose

    Expression of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBa.PEX 1-42-tdTM-FKBP

    Plasmid

    #224415

    Purpose

    Mammalian expression of Pex3

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    Pex3 (Pex3 Mouse)
    

    Use

    Tags

    tdTom-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPP4

    Plasmid

    #209966

    Purpose

    Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P40)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLP-EV

    Plasmid

    #209973

    Purpose

    Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    CDS (mock)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SL3-MS2-gRNA GG backbone (MOBE3-4)

    Plasmid

    #219949

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-SL3 (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 3'-com-gRNA GG backbone (MOBE1-4)

    Plasmid

    #219947

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-com-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3'MS2-gRNA GG backbone (MOBE1-2)

    Plasmid

    #219948

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge