We narrowed to 309,096 results for: Des
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Plasmid#118723PurposeThe donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry
Plasmid#69232PurposeWild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)DepositorInsertSpCas9
UseCRISPRTags2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…ExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PBD-GFP-U6ac-rac2 guides
Plasmid#168251Purpose"label active Rac under Rac2 neutrophil-specific knockout background"DepositorInsertPBD GFP
UseZebrafish expressionPromoterLyzCAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides
Plasmid#168254Purpose"label stable F-actin under Rac2 neutrophil-specific knockout background"DepositorInsertUtrophin-GFP
UseZebrafish expressionPromoterLyzCAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K
Plasmid#183357PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4 with ICE activity (CASP4 Human)
UseRetroviralTagsMycMutationANR261ENP, D315H, M318K, T352KPromoterMESVAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PBD-GFP-U6ac-ctrl guides
Plasmid#168252Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertPBD GFP
UseZebrafish expressionPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides
Plasmid#168253Purpose"label stable F-actin; control for neutrophil-specific knockout"DepositorInsertUtrophin-GFP
UseZebrafish expressionPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
ExpressionWormMutationNot applicableAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based no force control)
Plasmid#118722PurposeThe donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2
Plasmid#69228PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cellDepositorInsertTS2 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3
Plasmid#69229PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cellDepositorInsertTS3 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4
Plasmid#69230PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cellDepositorInsertTS4 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP
Plasmid#65846PurposeMammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP
Plasmid#65847PurposeMammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala7-TEV-YFP-Flag
Plasmid#41013DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
TagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1189-1192 to AlanineAvailable SinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag
Plasmid#41012DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
TagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1185-1188 to AlanineAvailable SinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag
Plasmid#41011DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
TagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1183-1186 to AlanineAvailable SinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR-ON VIPER CAR (anti-CD19)-final design
Plasmid#192117PurposeLentiviral deliviery of ON VIPER CAR (anti-CD19)-final designDepositorInsertpHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)
UseLentiviralAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK Lentivirus Multi Cassette Destination Vector (pAN2424)
Plasmid#194221PurposeBackbone for constructing multi casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I affinity mutant (F40-based tension sensor)
Plasmid#119190PurposeThe affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.DepositorInserthuman Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only