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NK591

Plasmid

#109080

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mTFP1

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1331 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]

Plasmid

#107854

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

W385G in splicing reporter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only
NK613

Plasmid

#109091

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mCherry_SV40 NLS

Use

Choanoflagellate expression

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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NK648

Plasmid

#109104

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

3'UTR EFL

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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NK597

Plasmid

#109086

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

tdTomato

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK595

Plasmid

#109083

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

EGFP

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK585

Plasmid

#109079

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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NK609

Plasmid

#109078

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK588

Plasmid

#109077

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

pActAvrII_nanoluc

Use

Choanoflagellate expression

Mutation

Eliminate NcoI site in the promoter

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK606

Plasmid

#109076

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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NK587

Plasmid

#109075

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

nanoluc

Use

Choanoflagellate expression

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1337 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G309S]

Plasmid

#107849

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

G309S in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

Plasmid

#107847

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All asparagine to glutamine in splicing reporter

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

Plasmid

#107843

Purpose

To test the effect of sequence on TDP43 splicing activity

Depositor

Rajat Rohatgi

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All phenylalanine to serine in splicing reporter

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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p5lysAsfGFP

Plasmid

#92025

Purpose

sfGFP reporter plasmid with lysA TALE binding site in its promoter

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Insert

sfGFP

Use

TALEN

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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p5sucAmCherry

Plasmid

#92026

Purpose

mCherry reporter plasmid with sucA TALE binding site in its promoter

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Insert

mCherry

Use

TALEN

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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FoRC.PGm

Plasmid

#84586

Purpose

Drosophila P-element transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.

Depositor

Francesca Pignoni

Article

Zhou et al Genesis. 2016 Oct 3. doi: 10.1002/dvg.22

Type

Empty backbone

Expression

Insect

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJK289

Plasmid

#71553

Purpose

Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)

Depositor

T. J. Kappock

Article

Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

Insert

alanine racemase

Expression

Bacterial

Mutation

changes lysine-39 to alanine (please see deposito…

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCH101

Plasmid

#72308

Purpose

Prhl/lac-w reporter plasmid

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

modified YFP

Expression

Bacterial

Mutation

T203Y sfGFP->YFP

Promoter

Prhl/lac-w

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSGE_895_Nhe2_EcR-to-gl

Plasmid

#71184

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

895_Nhe2_EcR-to-gl

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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