We narrowed to 5,703 results for: Feng Zhang

1. lenti-UCOE-dCas9-BFP-VP64

   Plasmid

   #196717

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213969

   Purpose

   AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CBA-dt/PGK-CFTR-⌀

   Plasmid

   #163923

   Purpose

   Expresses human CFTR.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   CFTR (CFTR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF6-CSPG4-myc-his

   Plasmid

   #69037

   Purpose

   Expression of CSPG4 in mammalian cells

   Depositor

   Wensheng Wei

   Article

   Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10

   Insert

   CSPG4 (CSPG4 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1 alpha

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-G88P7-rM3Ds-2A-EYFP

   Plasmid

   #213970

   Purpose

   AAV vector with G88P7 promoter in order to induce robust expression of rM3Ds in MSNs

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Insert

   rM3Ds
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   G88P7

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-GFAP-NLS-CasRx-NLS-FLAG

   Plasmid

   #154000

   Purpose

   Express CasRx for AAV package

   Depositor

   Hui Yang

   Article

   Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

   Insert

   CasRx
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   GFAP

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-G88P3-HA-hM3Dq

   Plasmid

   #213972

   Purpose

   AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

   Depositor

   Zhonghua Lu

   Article

   Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

   Insert

   hM3D (Gq)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   G88P3

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

   Plasmid

   #194755

   Purpose

   Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

   Depositor

   Chaoyang Li

   Article

   Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

   Insert

   PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

   Plasmid

   #224388

   Purpose

   Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   WD 7 domain deleted (aa 286-323)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

    Plasmid

    #224387

    Purpose

    Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

    Depositor

    Junjie Chen

    Article

    Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

    Insert

    GNB1L (GNB1L Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    WD 6 domain deleted (aa 242-282)

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

    Plasmid

    #224385

    Purpose

    Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

    Depositor

    Junjie Chen

    Article

    Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

    Insert

    GNB1L (GNB1L Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    WD 4 domain deleted (aa 153-195)

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459_GNB1L_gRNA

    Plasmid

    #224377

    Purpose

    Targets GNB1L for dTAG C terminal knock-in cell lines

    Depositor

    Junjie Chen

    Article

    Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

    Insert

    GNB1L (GNB1L Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

    Plasmid

    #194753

    Purpose

    Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchor

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Insert

    PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194752

    Purpose

    Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchor

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Insert

    PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2-sgCTL

    Plasmid

    #234771

    Purpose

    Non Targeting Control

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Non Targeting Control sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas12Max

    Plasmid

    #195334

    Purpose

    vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized Cas12Max
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    N243R

    Promoter

    CBh, CMV, hU6

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. xCas12i

    Plasmid

    #195333

    Purpose

    vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized xCas12i
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, CMV, hU6

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. DHFR-SpCas9-DHFR

    Plasmid

    #85447

    Purpose

    Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-SpCas9-DHFR
    

    Use

    AAV

    Tags

    Destabilized domain (C-terminus version) of E.col…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. gh24

    Plasmid

    #106697

    Purpose

    expression of gRNA targeting POMT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    POMT2 (POMT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

20. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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