We narrowed to 7,548 results for: ski

1. pEND-304_Cacnes_tetR

   Plasmid

   #225619

   Purpose

   C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   TetR
   

   Use

   Synthetic Biology

   Tags

   6xHis and mCherry-V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEND-375_Cacnes_phlF

   Plasmid

   #225620

   Purpose

   C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   PhlF
   

   Use

   Synthetic Biology

   Tags

   6xHis and mCherry-V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEND-226_Cacnes_mCitrine

   Plasmid

   #225613

   Purpose

   Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   mCitrine
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV.CBA.YFP.miR-E_shControl

   Plasmid

   #180386

   Purpose

   Producing AAV that encodes negative control shRNA with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   shControl
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR v2-sgDHODH-2

   Plasmid

   #186023

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CR1-isoformB-CD4d3+4-bio

   Plasmid

   #73099

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CR1-isoformB with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   CR1 isoform B (CR1 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector

   Plasmid

   #172514

   Purpose

   Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV NucleoBAS

   Plasmid

   #62861

   Purpose

   Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

   Depositor

   Stan van de Graaf

   Article

   van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

   Insert

   NucleoBAS
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. SFFV-AID-CTERM-GFP

    Plasmid

    #185768

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    William Sellers, Alessandra Ianari

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, AID

    Expression

    Mutation

    none

    Promoter

    SFFV

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV HA hRB delta CDK + T373

    Plasmid

    #58912

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T373 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. sh-SRF

    Plasmid

    #100797

    Purpose

    expression of shRNA targeting SRF

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

    Insert

    rat SRF
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-FH-Nsp14

    Plasmid

    #157718

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA5-FRT-TO-Nsp13

    Plasmid

    #157712

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp13 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. VZV ORF 60 (gL)

    Plasmid

    #64082

    Purpose

    eukaryotic expression plasmid for VZV glycoprotein L

    Depositor

    Randall Cohrs

    Article

    Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

    Insert

    VZV glycoprotein L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE with intron

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-Nsp10-HF

    Plasmid

    #157708

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp10-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAAV-CaMKIIa-HA-eLACCO2.1-NGR

    Plasmid

    #207989

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Yuki Kambe, Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-gfaABC1D-HA-deLACCO1-NGR

    Plasmid

    #208099

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Marie-Eve Paquet, Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VZV ORF 37(gH)

    Plasmid

    #64081

    Purpose

    eukaryotic expression plasmid for VZV glycoprotein H

    Depositor

    Randall Cohrs

    Article

    Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

    Insert

    VZV glycoprotein H
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE with intron

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCS2+-HypocratesCS

    Plasmid

    #161960

    Purpose

    Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

    Depositor

    Dmitry Bilan

    Article

    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

    Insert

    Hypocrates
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 355 to Serine

    Promoter

    CMV, SP6

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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