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pNBU2_erm_P1T_DP-GH023 - NanoLuc

Plasmid

#117727

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Expression

Bacterial

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45565

Purpose

Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pmEGFP-ADRA2A

Plasmid

#190753

Purpose

Expression of ADRA2A with mEGFP tag on N-terminus.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

ADRA2A (ADRA2A Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pnCas9-AID

Plasmid

#196242

Purpose

Plasmid expressing the nCas9-AID fusion

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Insert

nickase Cas9 fused to AID

Use

CRISPR

Expression

Bacterial

Promoter

tac promoter

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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p458 VQR

Plasmid

#101727

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Expression

Mammalian

Mutation

VQR

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pYTK-DN6

Plasmid

#180287

Purpose

HygR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - HygR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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p2,0_3E5E_luciferase

Plasmid

#69358

Purpose

Encodes for Ebola Minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

3E5E luciferase minigenome

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBad-Int Set Flipper

Plasmid

#38209

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Insert

Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Tags

His

Promoter

pBAD/AraC

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAM236 Ubc4

Plasmid

#226361

Purpose

Expression of yeast Ubc4

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

Ubc4 (UBC4 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Phsp-16 cz::caspase-3 (p17) [TU#823]

Plasmid

#16086

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz :: caspase-3 (p17) (CASP3 Human)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pYTK-DN5

Plasmid

#180286

Purpose

NatR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - NatR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRST11B-AS3_4

Plasmid

#102789

Purpose

Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Inserts

tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
suppressor tRNA

Expression

Bacterial

Available Since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b-pchG WT

Plasmid

#87107

Purpose

expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

Insert

pchG WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AT110.i1

Plasmid

#135481

Purpose

E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

Depositor

Andrew Kruse

Article

Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

Insert

Nanobody AT110.i1

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAS3-rig-3P::AI::lgc-47::SL2::GFP::unc-54-3'UTR

Plasmid

#225924

Purpose

Expressing LGC-47 in AVA neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

rig-3

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB701NL3F10H

Plasmid

#141288

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO MycLAP-STIL

Plasmid

#80266

Purpose

mammalian expression plasmid for MycLAP-STIL

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

Myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pgRNA-R-gfp

Plasmid

#220927

Purpose

Expresses gfp using J23119 promoter and pgRNA_AT backbone

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Insert

superfolder GFP

Tags

FLAG

Expression

Bacterial

Promoter

J23119

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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