We narrowed to 15,798 results for: pcDNA

1. Venus-G protein Beta2 in pcDNA3.1

   Plasmid

   #42182

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   G protein Beta 2 (GNB2 Human)
   

   Use

   Tags

   Venus(156-239)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-H2A-Akt-STOPS

   Plasmid

   #175010

   Purpose

   Akt-STOPS targeted to the nucleus via histone H2A.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   H2A-mCherry-Akt-STOPS
   

   Use

   Tags

   Histone H2A and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/TO-N-mEGFP-PARP6

   Plasmid

   #178005

   Purpose

   Expresses N-terminally mEGFP-tagged PARP6

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP6 (PARP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT/TO-N-mEGFP-PARP15

   Plasmid

   #178000

   Purpose

   Expresses N-terminally mEGFP-tagged PARP15

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP15 (PARP15 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5-FRT/TO-N-mEGFP-PARP11

   Plasmid

   #177998

   Purpose

   Expresses N-terminally mEGFP-tagged PARP11

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP11 (PARP11 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-sACt-mCherry-NES

   Plasmid

   #138215

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   sACt-mCherry-NES (Adcy10 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-N-Myc_DED/AAA USP7

   Plasmid

   #131253

   Purpose

   Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert USP7 aspart…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5078 pcDNA4c hBrd4 (1-470)

   Plasmid

   #14454

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (1-470) (BRD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   amino acids 1-470

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5-FRT-TO-EGFP-AID

   Plasmid

   #80075

   Purpose

   Mammalian expression vector for N-terminal EGFP-AID tagging

   Depositor

   Andrew Holland

   Insert

   EGFP-AID
   

   Use

   Tags

   AID and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PERK 3: PERK.dC.9E10.pCDNA

    Plasmid

    #21816

    Depositor

    David Ron

    Article

    Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

    Insert

    PERK (C-term deleted) (Eif2ak3 Mouse)
    

    Use

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    amino-acids 582-1081 deleted

    Promoter

    Available since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-Myc-His RTN3A1

    Plasmid

    #31087

    Depositor

    Jimtong Horng

    Article

    Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

    Insert

    reticulon 3 (RTN3 Human)
    

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)/CUG-nLuc-3XFLAG

    Plasmid

    #127300

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid

    #182334

    Purpose

    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    DrFLINC-AKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 Flag MKK7b1(glu)

    Plasmid

    #14673

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(Glu) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-269Glu Thr-273Glu

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3 Flag MKK3b(Glu)

    Plasmid

    #50449

    Purpose

    activates p38 in the signaling pathway

    Depositor

    Roger Davis

    Article

    Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

    Insert

    MKK3b (MAP2K3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 218 to Glutamic Acid, changed Thre…

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA5/FRT/TO-AT1R-APEX

    Plasmid

    #96847

    Purpose

    For expression of AT1R-APEX in mammalian cells

    Depositor

    Andrew Kruse

    Article

    Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

    Insert

    AT1R-APEX (AGTR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-MTS-CA-c-Src-FLAG

    Plasmid

    #44654

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed Tyr 530 to Phe

    Promoter

    CMV promoter

    Available since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-HA-Akt1-aa1-149

    Plasmid

    #73410

    Purpose

    Expresses HA tagged aa1-149 of AKT1

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Expresses aa 1-149 only of AKT1

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5/FRT/TO-FNTA-FLAG

    Plasmid

    #86858

    Purpose

    To express FNTA with a FLAG tag at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    FNTA (FNTA Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The sequence of the insert is isoform 2, which di…

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Yellow Cameleon-Nano50/pcDNA3

    Plasmid

    #51964

    Purpose

    mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge