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pORANGE GFP-Grin1 KI #2

Plasmid

#139658

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-RandE3

Plasmid

#79870

Purpose

Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-RandE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

Beta-actin

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-HRAS

Plasmid

#80604

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

HIV-1 p55 GAG

Expression

Mammalian

Promoter

SRalpha

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-C–dSpCas9

Plasmid

#157836

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE WASF1-GFP KI

Plasmid

#131502

Purpose

Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Wasf1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb2-GFP KI

Plasmid

#139661

Purpose

Endogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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Dimer-FRB

Plasmid

#239847

Purpose

Expresses a de novo dimer fused to FRB

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

Dimer-FRB

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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3mer-FKBP

Plasmid

#239848

Purpose

Expresses a de novo 3mer fused to FKBP

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

3mer-FKBP

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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4mer-FKBP

Plasmid

#239849

Purpose

Expresses a de novo 4mer fused to FKBP

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

4mer-FKBP

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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6mer-FKBP

Plasmid

#239851

Purpose

Expresses a de novo 6mer fused to FKBP

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

6mer-FKBP

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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12mer-FKBP

Plasmid

#239852

Purpose

Expresses a de novo 12mer fused to FKBP

Depositor

Cole DeForest

Article

Gregorio et al Cell Biomaterials, 2025

Insert

12mer-FKBP

Tags

6X His Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMWCAVI_H6BAP-OROV_N

Plasmid

#240162

Purpose

Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…

Expression

Bacterial

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPTH OROV N

Plasmid

#229663

Purpose

Express Oropouche virus BeAn 19991 nucleoprotein with N-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-PB2_Lock1-I436C+S993C

Plasmid

#182879

Purpose

Lentivirus for expression of Plexin-B2 locked ring mutant

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Mutation

changed Isoleucine 436 to Cysteine and Serine 993…

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-PB2_Lock2-I436C+T1051C

Plasmid

#182880

Purpose

Lentivirus for expression of Plexin-B2 locked ring mutant

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Mutation

changed Isoleucine 436 to Cysteine and Threonine …

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-EGFP

Plasmid

#80605

Purpose

Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

EGFP

Expression

Mammalian

Promoter

SRalpha

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV MCS mCherry-KASH

Plasmid

#139654

Purpose

Cloning template for AAV-based ORANGE knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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