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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,042 results for: sms

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Showing: 4541 - 4560 of 8042 results

  1. pαH-S-GSAS-B.1.351

    Plasmid
    #171749
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171749-plasmid-map-sequence-id-337764

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  2. pαH-S-GSAS-D614G-E484K

    Plasmid
    #171751
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-E484K variant
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-D614G-E484K variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171751-plasmid-map-sequence-id-337763

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  3. pαH-S-GSAS-D614G-K417N

    Plasmid
    #171752
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N variant
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-D614G-K417N variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171752-plasmid-map-sequence-id-337762

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  4. pαH-S-GSAS-D614G-K417N-E484K-N501Y

    Plasmid
    #171750
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N-E484K-N501Y
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-D614G-K417N-E484K-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171750-plasmid-map-sequence-id-337761

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  5. pαH-S-GSAS-D614G-I692V

    Plasmid
    #171746
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-I692V variant
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-I692V variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171746-plasmid-map-sequence-id-337719

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  6. pαH-S-GSAS-D614G-Y453F

    Plasmid
    #171745
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-Y453F variant
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-Y453F variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171745-plasmid-map-sequence-id-337717

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  7. pαH-S-GSAS-D614G-deltaFV

    Plasmid
    #171743
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171743-plasmid-map-sequence-id-337714

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  9. CFP(1-158)-gamma-1 in pcDNAI/Amp

    Plasmid
    #55615
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP(1-158)-gamma-1 (GNGT1 Aequorea victoria, Bovine)
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-1 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55615-plasmid-map-sequence-id-115069

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  10. YFP(159-238)-gamma-10 in pcDNAI/Amp

    Plasmid
    #55192
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-10. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    YFP-(159-238)-gamma-10 (GNG10 Aequorea victoria, Human)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-10 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55192-plasmid-map-sequence-id-115049

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  11. YFP(159-238)-gamma-12 in pcDNAI/Amp

    Plasmid
    #55194
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-12. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    YFP(159-238)-gamma-12 (GNG12 Aequorea victoria, Human)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-12 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55194-plasmid-map-sequence-id-115063

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  12. CFP(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55616
    Purpose
    An amino-terminal CFP fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP (1-158)-gamma-2 (Gng2 Aequorea victoria, Mouse)
    Tags
    CFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-2 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55616-plasmid-map-sequence-id-115112

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  14. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183515-plasmid-map-sequence-id-362649

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  15. pINTO-N3::hTET3 HxD

    Plasmid
    #136365
    Purpose
    Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells
    Depositor
    Roberto Bonasio
    Publication
    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET3 (TET3 Human)
    Tags
    2xStrepTagII, FLAG, and HA
    Expression
    Mammalian
    Mutation
    HxD (H1077Y, D1079A)
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136365-plasmid-map-sequence-id-271993

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  19. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Publication
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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Showing: 4541 - 4560 of 8042 results