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pCon-ICl_Con-ICr

Plasmid

#192214

Purpose

Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-C Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IIAl_Con-IIAr

Plasmid

#192215

Purpose

Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus II-A Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX1-IFl_Con-IFr

Plasmid

#192216

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X1)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX2-IFl_Con-IFr

Plasmid

#192217

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X2)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pADSS-EGFP

Plasmid

#228197

Purpose

expressed human ADSS with an EGFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

Zhao et al J Biol Chem. 2015 Mar 13;290(11):6705-13

Insert

ADSS (ADSS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-NRG1-ScNeo

Plasmid

#209911

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Tags

mNeonGreen and mScarlet

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-mNeonGreen

Plasmid

#209912

Purpose

To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-mNeonGreen (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EREG-mNeonGreen

Plasmid

#209914

Purpose

To monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EREG-mNeonGreen (EREG Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbleo-TGFa-ScNeo

Plasmid

#209904

Purpose

To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-ScNeo (TGFA Human)

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-TGFa-ScNeo

Plasmid

#209908

Purpose

To monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-ScNeo (TGFA Human)

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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(attB) tdMCP-tagged BRaf

Plasmid

#226165

Purpose

Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

3xFlag-tdMCP-BRaf (BRAF Human)

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP and IRES-mCherry-P2A-PuroR

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC017

Plasmid

#226622

Purpose

pLenti wt hTdT template for libraries 1-2

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

Terminal deoxynucleotidyl transferase (DNTT Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

synonymous mutation in D2, synonymous mutation in…

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_SAT1 (human)

Plasmid

#220477

Purpose

heterologous expression of SSAT1 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-C1-Nir1-631-894

Plasmid

#220089

Purpose

Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM3 (PITPNM3 Human)

Tags

NeonGreen-GGSGG

Expression

Mammalian

Mutation

amino acids 631-894

Promoter

CMV

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-N1-Nir1-613-630

Plasmid

#220088

Purpose

N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM3 (PITPNM3 Human)

Tags

GGSGG-NeonGreen

Expression

Mammalian

Mutation

amino acids 613-630

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-F14F15S-jGCaMP7s

Plasmid

#178514

Purpose

AAV vector for Flpo recombinase dependent jGCaMP7s

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

jGCaMP7s

Use

AAV

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-mCre

Plasmid

#178515

Purpose

AAV vector for mCre recombinase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCre

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_G1A-T2A_R

Plasmid

#200183

Purpose

WT PA14 I-F Leader and G1A-T2A Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A)

Expression

Bacterial

Mutation

G1A-T2A in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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WT_IFL_G1A-T2A-A28C_R

Plasmid

#200185

Purpose

WT PA14 I-F Leader and G1A-T2A-A28C Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)

Expression

Bacterial

Mutation

G1A-T2A-A28C in the I-F repeat

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-457

Plasmid

#200213

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 H25A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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