We narrowed to 88,195 results for: MAL

1. pAB1676 REPAIR.t1

   Plasmid

   #176319

   Purpose

   CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Inserts

   human codon optimized Cas13bt1 (catalytically inactivated)
   huADAR2dd(E488Q) (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   HIV NES

   Expression

   Mammalian

   Mutation

   E488Q, only the deaminase domain (aa 276-702 are …

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

   Plasmid

   #64219

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E1B

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

   Plasmid

   #114086

   Purpose

   CAG promoter expression plasmid for human codon optimized LbBE1.4

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
   

   Use

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)

   Plasmid

   #114095

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-RR (E174R/S542R/K607R)
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R, S542R and K607R

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-Myc-FBW7 Delta F-Box

   Plasmid

   #197452

   Purpose

   The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FBW7 Fbox deleted.

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa-FRB-3myc-KIF1B beta tail 387-1770

   Plasmid

   #64287

   Purpose

   KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   KIF1B beta (Kif1b Mouse, Rat)
   

   Use

   Tags

   3myc and FRB

   Expression

   Mammalian

   Mutation

   aa 387-1770, small portion of 5'end matches …

   Promoter

   chicken Beta Actin

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP.mIKKβ

   Plasmid

   #74412

   Purpose

   Expresses mouse eGFP-IKKβ in mammalian cells

   Depositor

   Georgios Stathopoulos

   Article

   Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

   Insert

   mouse IKKβ (Ikbkb Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLZRS-ER-Rac1-Q61L-shortlinker

   Plasmid

   #128584

   Purpose

   For mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   Modified hormone binding domain of ER alpha

   Expression

   Mammalian

   Mutation

   Changed Glutamine61 to Leucine in the RAC1 moiety.

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLZRS-ER-Rac1-P29S-shortlinker

   Plasmid

   #128583

   Purpose

   For mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHT

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   Modified hormone binding domain of ER alpha

   Expression

   Mammalian

   Mutation

   Changed Proline29 to Serine in the RAC1 moiety.

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE_hygro_mRFP1_NRF2(C506S, L4A)

    Plasmid

    #136580

    Purpose

    mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (C506S, Leuzip mut) (NFE2L2 Human)
    

    Use

    Retroviral

    Tags

    N-mRFP1-FLAG

    Expression

    Mammalian

    Mutation

    The C506S mutation (C513S in the actual sequence …

    Promoter

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-N2H-F2-empty vector

    Plasmid

    #125552

    Purpose

    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment2 alone without any attached prot…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CMV-PdCO-EGFP

    Plasmid

    #198512

    Purpose

    Expresses optimized PdCO in frame with EGFP under control of CMV promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid

    #114094

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-RR (S542R/K607R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    S542R and K607R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-N2H-F1-empty vector

    Plasmid

    #125551

    Purpose

    N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1)

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 alone without any attached prot…

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP.mIKKα

    Plasmid

    #74413

    Purpose

    Expresses mouse eGFP-IKKα in mammalian cells

    Depositor

    Georgios Stathopoulos

    Article

    Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

    Insert

    mouse IKKα (Chuk Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid

    #114092

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (S542R/K548V/N552R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    S542R, K548V and N552R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

    Plasmid

    #114080

    Purpose

    CAG promoter expression plasmid for human codon optimized AsBE1.4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid

    #114091

    Purpose

    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R)
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    E174R and S542R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid

    #114079

    Purpose

    CAG promoter expression plasmid for human codon optimized AsBE1.1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Mirror GFP[ENE(C8351G)-mascRNA(mut 8356-8370)](pAVA3871)

    Plasmid

    #239352

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(C8351G)-mascRNA(mut 8356-8370) reporter.

    Depositor

    Andrei Alexandrov

    Article

    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

    Insert

    MALAT1 ENE(C8351G)-mascRNA(mut 8356-8370)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MALAT1 ENE(C8351G)-mascRNA(mut 8356-8370: ctacgac…

    Promoter

    Available since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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