We narrowed to 118,634 results for: MPI;

1. pSpCas9(BB)-2A-Hygro

   Plasmid

   #127763

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-puro-cOVA

   Plasmid

   #135073

   Purpose

   Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

   Depositor

   Maria Castro

   Insert

   cOVA (OVAL Chicken)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti Lifeact-mTagBFP2 PuroR

   Plasmid

   #101893

   Purpose

   Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Promoter

   CMV immediate early

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-Hygro

   Plasmid

   #139462

   Purpose

   Lentiviral vector with gRNA scaffold and hygromycin selectable marker

   Depositor

   Caroline Goujon

   Insert

   no sgRNA inserted; resistance gene: hygroR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 gfaABC1D-cyto-GCaMP8f

   Plasmid

   #186050

   Purpose

   AAV-mediated expression of GCaMP8f under the control of GfaABC1D promoter

   Depositor

   Loren Looger

   Insert

   jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5B-TGFbeta receptor II wt (N-term HA)

   Plasmid

   #24801

   Depositor

   Jeff Wrana

   Insert

   TGFbeta receptor II (TGFBR2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pOpen-SfiIR (cDNA)

   Plasmid

   #165528

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   SfiI (used to create cDNA libraries)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3-mRFP

   Plasmid

   #13032

   Purpose

   Mammalian expression vector for cloning your gene fused to mRFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Monomer Red Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. HRE:GFP-pGK:Cre

   Plasmid

   #68552

   Purpose

   Lentivirus expressing hypoxia-responsive GFP reporter and Cre

   Depositor

   Tyler Jacks

   Inserts

   Green Fluorescent Protein
   Cre
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   HRE and pGK

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOpen-T5gene122

    Plasmid

    #165544

    Purpose

    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    T5 DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLentiV2-dCas9-VP64

    Plasmid

    #141104

    Purpose

    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

    Depositor

    Igor Ulitsky

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pExp-NusA

    Plasmid

    #112571

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-YFP

    Plasmid

    #13033

    Purpose

    Mammalian expression vector for cloning your gene fused to YFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Yellow fluorescent protein
    

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 neo

    Plasmid

    #13425

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.

    Depositor

    Sheila Stewart

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Dec. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOpen-P45

    Plasmid

    #165570

    Purpose

    Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    P45
    

    Use

    Synthetic Biology

    Available Since

    Aug. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. PB CAG-L-3xSV40pA-L-td-sfGFP

    Plasmid

    #133574

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1-copGFP-T2A-Puro

    Plasmid

    #72263

    Purpose

    Express copGFP and puromycin resistance gene under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    copGFP-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    elongation factor-1

    Available Since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid

    #115519

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    ccdB-sgRNA scaffold
    

    Use

    CRISPR

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FU-H2B-GFP-IRES-Puro

    Plasmid

    #69550

    Purpose

    Lentiviral, selectable fluorescent GFP tagging of nuclei.

    Depositor

    Charles Gersbach

    Insert

    Histone 2B - green fluorescent protein fusion
    

    Use

    Lentiviral

    Tags

    GFP fusion

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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