We narrowed to 5,114 results for: mcs

1. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

   Plasmid

   #234952

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234953

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDH-EF1-IRES-BSD

   Plasmid

   #209751

   Purpose

   Lentiviral transfer plasmid to express the blasticidin resistance gene

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV pmSyn1-EBFP-Cre

   Plasmid

   #51507

   Purpose

   Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Has Service

   AAV5

   Insert

   EBFP-Cre
   

   Use

   AAV

   Promoter

   pmSyn1

   Available Since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-puro-N-3HA-SREBP1-C-Flag

   Plasmid

   #134286

   Purpose

   Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP1 (Srebf1 Mouse)
   

   Use

   Lentiviral

   Tags

   3x HA and Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP1 isoform a precursor

   Promoter

   EF1a

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234954

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-puro-N-3HA-SREBP2-C-Flag

   Plasmid

   #134287

   Purpose

   Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP2 (Srebf2 Mouse)
   

   Use

   Lentiviral

   Tags

   3x HA and Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP2 precursor

   Promoter

   CMV

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

   Plasmid

   #65458

   Purpose

   Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   tTA2
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65455

   Purpose

   Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-STAT-CA

    Plasmid

    #195576

    Purpose

    Expresses STAT3 constitutively active mutant

    Depositor

    Kevin Park

    Article

    Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

    Insert

    Signal transducer and activator of transcription 3 (Stat3 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    changed Alanine 662 to Cysteine and Asparagine 66…

    Promoter

    CMV enhancer and promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-EF1a-extGluc-mKate-NGFR

    Plasmid

    #104831

    Purpose

    extGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detection

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    mKate
    NGFR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV pTRE-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65449

    Purpose

    Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    tetO

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-CuO-V5 CASC3 siRes 1-480 WT

    Plasmid

    #158541

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 1-480, C-terminal deletion

    Promoter

    CMV

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1a-extGluc-NGFR

    Plasmid

    #104832

    Purpose

    extGluc luciferase for invivo Imaging, NGFR for flourescent detection or separation

    Depositor

    Irmela Jeremias

    Article

    Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

    Inserts

    extGLuc
    NGFR
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid

    #137722

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Truncated at amino acid 1328

    Promoter

    Tight TRE promoter

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

    Plasmid

    #158542

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 1-480, C-terminal deletion, F188D, W2…

    Promoter

    CMV

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-CuO-V5 CASC3 siRes 110-480 WT

    Plasmid

    #158543

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 110-480, C-terminal deletion

    Promoter

    CMV

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA

    Plasmid

    #65453

    Purpose

    Can be used to generate AAV virus that will express EGFP from the tetO promoter under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV1

    Insert

    EGFP
    

    Use

    AAV

    Promoter

    tetO

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

    Plasmid

    #194755

    Purpose

    Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Insert

    PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV phSyn1(S)-FlpE-bGHpA

    Plasmid

    #50364

    Purpose

    Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    FlpE recombinase gene
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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