We narrowed to 26,396 results for: pET

1. pETM11-6xHis-TEV-hORF1p_R261A

   Plasmid

   #202586

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM11-6xHis-TEV-hORF1p_StammerAAA

   Plasmid

   #202587

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM11-6xHis-TEV-hORF1p_StammerAEA

   Plasmid

   #202588

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Methionine 91 to Alanine, changed O…

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETSyt1C2AB(143-421)msGFP

   Plasmid

   #209010

   Purpose

   Recombinant protein expression of truncated syt1 tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 143-421 only

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETSyt1C2AB(80 JuxtaK-421)msGFP

   Plasmid

   #209011

   Purpose

   Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Tags

   6xHis and msfGFP

   Expression

   Bacterial

   Mutation

   aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-HypaSpCas9-NLS-6xHis

   Plasmid

   #207384

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PET-B-HeFSpCas9-NLS-6xHis

   Plasmid

   #207392

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-evoSpCas9-NLS-6xHis

   Plasmid

   #207389

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28 sf:em-blue-shift.1

    Plasmid

    #191928

    Purpose

    Encodes a GFP design, sf:em-blue-shift.1

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:em-blue-shift.1
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69P S72A T108V Y145M T167V V224I

    Promoter

    T7

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28b(+)/ADK C77S

    Plasmid

    #201409

    Purpose

    Overexpresses Escherichia coli adenylate kinase C77S

    Depositor

    Gary Pielak

    Article

    Brom et al Biomol NMR Assign. 2023 Aug 26. doi: 10.

    Insert

    Escherichia coli adenylate kinase C77S (adk Escherichia coli)
    

    Expression

    Bacterial

    Mutation

    changed cysteine 77 to serine

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET24-aCatenin(82-906)-His6

    Plasmid

    #203332

    Purpose

    Bacterial expression of wild-type alpha-catenin

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 82-906

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-51b(+) ChemoB-CaM

    Plasmid

    #193812

    Purpose

    Protein production of ChemoB-CaM in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-CaM
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EBFP2: N39Y, V206K

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-51b(+) ChemoC-CaM

    Plasmid

    #193813

    Purpose

    Protein production of ChemoC-CaM in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoC-CaM
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-51b(+) ChemoY-CaM

    Plasmid

    #193814

    Purpose

    Protein production of ChemoY-CaM in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoY-CaM
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Venus:A206K

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-51b(+) ChemoR-CaM

    Plasmid

    #193815

    Purpose

    Protein production of ChemoR-CaM in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoR-CaM
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    mScarlet: D201K

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-TRE-YPet-WPRE

    Plasmid

    #193335

    Purpose

    Expresses YPet under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    YPet
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a LZ LED 4CA-FLAG

    Plasmid

    #199681

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED 4CA (GPR155 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    C595.604.629.638 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a LZ LED Y551A-FLAG

    Plasmid

    #199680

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED Y551A (GPR155 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Y551 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM-41P-DmGW182_744-817_N

    Plasmid

    #147064

    Purpose

    Bacterial Expression of DmGW182_744-817

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182_744-817 (gw Fly)
    

    Expression

    Bacterial

    Mutation

    one non silent mutations N799S and one silent mut…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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