We narrowed to 12,317 results for: 112

1. pBCL6.1.0-gDNA

   Plasmid

   #112485

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6

   Depositor

   Kevin White

   Insert

   BCL6 (BCL6 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1667

   Plasmid

   #112041

   Purpose

   CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   ERG5
   

   Tags

   V5-TEV-EGFP

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mScarlet-I-T2A-LSS-SGFP2

   Plasmid

   #112946

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Insert

   mScarlet-I and LSS-SGFP2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1 FRB-myc-RagC

   Plasmid

   #112741

   Purpose

   express FRB-myc-RagC

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   FRB-myc-RagC (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   FRB, myc

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(R49A)

   Plasmid

   #112726

   Purpose

   Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R49A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R49A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1660

   Plasmid

   #112034

   Purpose

   C-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEU

   Depositor

   Jonathan Weissman

   Article

   Costa et al Science. 2018 Jan 18. pii: science.aar36

   Insert

   IAA17
   

   Tags

   HA

   Expression

   Yeast

   Mutation

   only amino acids 71-114

   Promoter

   None

   Available Since

   Aug. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-APIC-LexAGAD

   Plasmid

   #112807

   Purpose

   gateway cloning destination vector to express enhancer driven LexAGAD in Drosophila

   Depositor

   Chun Han

   Article

   Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p415 Gcs1

   Plasmid

   #112646

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Gcs1
   

   Expression

   Yeast

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LifeAct-LSS-SGFP2-N1

   Plasmid

   #112938

   Purpose

   Visualization of F-actin

   Depositor

   Dorus Gadella

   Insert

   LifeAct
   

   Tags

   LSS-SGFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-2T-NM

    Plasmid

    #1127

    Depositor

    Susan Lindquist

    Article

    Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

    Insert

    NM (SUP35 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

    Plasmid

    #112212

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    N-terminus of dCas9 and DNMT3A (DNMT3A Human)
    

    Use

    AAV

    Mutation

    H840A,N863A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOP2HA

    Plasmid

    #112598

    Purpose

    Used for expression of protein in E. coli as non-cleavable C-terminal 8His-Avi tag fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His-Avi tag

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTFE3-donor

    Plasmid

    #112377

    Purpose

    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP

    Depositor

    Kevin White

    Insert

    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    

    Use

    CRISPR

    Mutation

    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

    Plasmid

    #112257

    Purpose

    Expresses RGS component of opto-RGS2 in mammalian cells

    Depositor

    Brian Chow

    Article

    Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

    Insert

    CRY2-mCherry-(GGGGS)3-hRGS2box
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CvcE in pGEX4t

    Plasmid

    #112977

    Purpose

    For protein expression and purification of Drosophila CvcE

    Depositor

    Terry Orr-Weaver

    Insert

    cycE (CycE Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACTB-(A)3x

    Plasmid

    #112057

    Purpose

    ACTB mRNA tagged with 3 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    3 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ACTB-(A)2x

    Plasmid

    #112056

    Purpose

    ACTB mRNA tagged with 2 copies of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    2 copies of Riboglow tag (variant A) after stop c…

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-3xflagGREB1b

    Plasmid

    #112222

    Purpose

    mammalian expression of flag tagged GREB1b

    Depositor

    Craig Burd

    Article

    Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

    Insert

    GREB1b (GREB1 Human)
    

    Tags

    3x flag

    Expression

    Mammalian

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-EF1aMS2p65HSF1hyg-W

    Plasmid

    #112920

    Purpose

    Lentiviral vector expressing MS2p65HSF1 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    MS2p65HSF1-T2Ahyg
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human EF1a promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)/Puro/FA/FFT-LL5BIP

    Plasmid

    #112830

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

    Insert

    LL5BIP (2410002F23Rik Mouse)
    

    Tags

    2xFlag-TEV

    Expression

    Mammalian

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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