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Plasmid#174793PurposeBacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)DepositorInsertPARP1-W318R (PARP1 Human)
UseTags6xHisExpressionBacterialMutationW318, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED18
Plasmid#15433DepositorInsertMED18 (MED18 Human)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A762V
Plasmid#174795PurposeBacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)DepositorInsertPARP1-A762V (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA762VPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED22
Plasmid#15432DepositorInsertMED22 (MED22 Human)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET32b-MED19
Plasmid#15438DepositorInsertMED19 (Med19 Mouse)
UseTagsHis and TrxExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED30
Plasmid#15431DepositorInsertMED30 (Med30 Mouse)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED31
Plasmid#15437DepositorInsertMED31 (Med31 Mouse)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Med18 pBacPAK8
Plasmid#15371DepositorInsertMed18 (MED18 Human)
UseBaculovirus constructionTagsFLAG and HisExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Med11 pBacPAK8
Plasmid#15373DepositorInsertMed11 (Med11 Mouse)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc-Med22 pBacPAK8
Plasmid#15374DepositorInsertMed22 (MED22 Human)
UseBaculovirus constructionTagsmycExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc-Med31 pBacPAK8
Plasmid#15375DepositorInsertMed31 (Med31 Mouse)
UseBaculovirus constructionTagsmycExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc-Med19 pBacPAK8
Plasmid#15376DepositorInsertMed19 (Med19 Mouse)
UseBaculovirus constructionTagsmycExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DXO
Plasmid#177993PurposeFor purification of N-terminally His-tagged DXO from bacteriaDepositorInsertDXO (DXO Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1-L713F
Plasmid#173937PurposeBacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 1-96; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3-L713F
Plasmid#173934PurposeBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn3-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 214-373; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G871L
Plasmid#174798PurposeBacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-G871L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationG871L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A870L
Plasmid#174797PurposeBacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-A870L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA870L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-K893I
Plasmid#174799PurposeBacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)DepositorInsertPARP1-K893I (PARP1 Human)
UseTags6xHisExpressionBacterialMutationK893I, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-K77R
Plasmid#174813PurposeBacterial expression of inactive ALC1 (K77R disrupts ATP binding)DepositorInsertALC1-K77R (CHD1L Human)
UseTagsGSTExpressionBacterialMutationK77RPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-E175Q
Plasmid#174814PurposeBacterial expression of inactive ALC1 (E175Q disrupts ATP binding)DepositorInsertALC1-E175Q (CHD1L Human)
UseTagsGSTExpressionBacterialMutationE175QPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only