... miss your grandfather’s 100th birthday, as your mom will never forgive you… #3 Honor the work day and... no to some of those birthday parties. Tell your mom you can’t make it to dinner this week. One swim lesson...

... Genetic Perturbation Platform at the Broad. His mom always told him that he has the best words and fingers...

...Contains MoMLV gag and pol; use with envelope plasmid Weinberg 12371 pCL-Eco Packaging Contains MoMLV gag,...pol, and env Verma 35614 pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454 pCMV-VSV-G Envelope Envelope... 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized...resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression of hTERT for creation of immortalized...resistance and Neo resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses...MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian cells...

... or 70% ethanol Distilled water Disinfectant Thermometer Water bath weights and floats Reagents None needed... example, number of drops per liter. Place a thermometer in the water bath. Oftentimes, the water bath...bath will have a spot to secure the thermometer in place. Turn the water bath on and set the appropriate...

...’t until much later when, in the 1960s, Osamu Shimomura studied the bioluminescent properties observed...green light. To investigate this green glimmer, Shimomura collected many, many jellyfish specimens from ...when the Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for their work on GFP. This...

...offer two simple steps to welcome these unforeseen moments. Widening your search The first step towards inviting...breakthroughs often happen when we allow ourselves moments of release and mental freedom. Practical approaches...fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling in the...

...murine thymoma viral oncogene homolog 2 PKBB, PKBBETA, PRKBB, RAC-BETA AKT3 v-akt murine thymoma viral ...expansion. Symbol Name Synonyms AKT1 v-akt murine thymoma viral oncogene homolog 1 AKT, MGC99656, PKB, PKB-ALPHA...antigen. Symbol Name Synonyms AKT1 v-akt murine thymoma viral oncogene homolog 1 AKT, MGC99656, PKB, PKB-ALPHA...

...derived from the Moloney Murine leukemia virus (MoMLV) and consisted of two plasmids: the packaging plasmid...particularly for clinical development. In addition, MoMLV is inefficient at infecting non-dividing cells and...out to create a better, safer system. Unlike the MoMLV, lentiviruses, a separate genus of the Retroviridae...

...plasmids available from Addgene were derived from MoMLV (Moloney Murine Leukemia Virus) or MSCV (Murine ...these viruses were derived from different genomes (MoMLV and MSCV for γ-retrovirus; HIV for lentivirus). ...

...neo-selectable marker flanked by FRT sites) Mammalian Mombaerts 15509 pBS-M71-IRES-CreFNF M71 targeting vector...neo-selectable marker flanked by FRT sites) Mammalian Mombaerts 17408 Puro.Cre empty vector Cre, Puro resistance...

...upended our lives and took this daring risk? What moment changed our whole outlook on the possibilities ...employment?  It never happened – there was never a “moment”. But there were a lot of very small steps. Life...

... if you find yourself in this situation, take a moment to pause and ask your audience if they are following...access to a list of posters until the day-of, take a moment to step aside to read through the list at the session...

...endoplasmic reticulum. And graduate students, momentarily forgetting that the physical model is based on...instances of focused attention become magical teaching moments. The models function as thinking tools – eliciting...

...leadership skills that are just not being tapped at the moment? You can take this time to step up and develop ... know you possessed. Think about your shining moments. Recall a few times you really felt great about...

... Genetically encoded ratiometric fluorescent thermometer A highly-sensitive genetically encoded temperature...Temperature Imaging with a Genetically Encoded Nanothermometer. Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu...

...Complex Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma viral oncogene homolog FOXO Forkhead box O GBL/...

...below. Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma...

...Framed photos and slideshows shared some of the moments we captured over the years, including an album ...

...ourselves. Therefore, in this blog post we’re taking a moment to reach out to you, our readers, and ask you to...