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Plasmid#134804PurposeIPTG inducible expression of WT S tRNA scaffold tRNADepositorInsertWildtype S tRNA (decodes TCG)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-11
Plasmid#134803PurposeIPTG inducible expression of S-TAGA-Evo1 qtRNADepositorInsertWildtype S tRNA (decodes TCG)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xY-3
Plasmid#134808PurposeIPTG inducible expression of Y-TAGA-Evo1 qtRNADepositorInsertY-TAGA-Evo1 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xY-1
Plasmid#134807PurposeIPTG inducible expression of Y-TAGA qtRNADepositorInsertY-TAGA qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xW-1
Plasmid#134805PurposeIPTG inducible expression of W-TAGA qtRNADepositorInsertW-TAGA qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xW-3
Plasmid#134806PurposeIPTG inducible expression of W-TAGA-Evo1 qtRNADepositorInsertW-TAGA-Evo1 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xQ-1
Plasmid#134794PurposeIPTG inducible expression of Q-TAGA qtRNADepositorInsertQ-TAGA qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xQ-3
Plasmid#134795PurposeIPTG inducible expression of Q-TAGA-Evo1 qtRNADepositorInsertQ-TAGA-Evo1 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xQ-4
Plasmid#134796PurposeIPTG inducible expression of Q-TAGA-Evo2 qtRNADepositorInsertQ-TAGA-Evo2 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xR-1
Plasmid#134797PurposeIPTG inducible expression of R-TAGA qtRNADepositorInsertR-TAGA qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xR-3
Plasmid#134798PurposeIPTG inducible expression of R-TAGA-Evo1 qtRNADepositorInsertR-TAGA-Evo1 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xR-4
Plasmid#134799PurposeIPTG inducible expression of R-TAGA-Evo2 qtRNADepositorInsertR-TAGA-Evo2 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pED14xS-3
Plasmid#134801PurposeIPTG inducible expression of S-TAGA-Evo2 qtRNADepositorInsertS-TAGA-Evo2 qtRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJUMP42x-2A(sfGFP)
Plasmid#126986PurposeLevel 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP22x-1A(sfGFP)
Plasmid#126969PurposeLevel 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL1_35
Plasmid#199042PurposeLevel 1 plasmid, barcoded ORIDepositorInsertRK2 ORI plus NGS barcode 12
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_166
Plasmid#199098PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertRK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNDGG003
Plasmid#231316PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only