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pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#69138

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE

Tags

none

Expression

Mammalian

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85006

Purpose

For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#85038

Purpose

For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

FRT-stop-FRT

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

Plasmid

#85179

Purpose

For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) tagRFP

Expression

Mammalian

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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M50 Super 8x TOPFlash

Plasmid

#12456

Purpose

Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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pRetrosuper Myc shRNA

Plasmid

#15662

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Myc shRNA (MYC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pRetrosuper USP28 shRNA-1

Plasmid

#15663

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

USP28 shRNA (USP28 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR/pSUPER+ (575-1)

Plasmid

#17338

Purpose

Gateway entry vector for shRNA expression under H1 promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pRetrosuper Fbw7 shRNA-1

Plasmid

#15660

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Fbw7 shRNA (FBXW7 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pRetrosuper Fbw7 shRNA-2

Plasmid

#15661

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Fbw7 shRNA (FBXW7 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits
pRetroSuper-GFP Smad2

Plasmid

#15722

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad2 shRNA (SMAD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-GFP Smad4

Plasmid

#15724

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad4 shRNA (SMAD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pSuper mouse Dicer1

Plasmid

#14779

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pRetroSuper-GFP Smad3

Plasmid

#15723

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad3 shRNA (SMAD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pSuper mouse Drosha2

Plasmid

#14775

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (Drosha Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
PSuperPuroRetro-1660(hPot1)

Plasmid

#11135

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pRetroSuper shSmad4J hygro

Plasmid

#19151

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

small hairpin RNA against SMAD family member 4 (SMAD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only
pRetrosuper USP28 shRNA-3

Plasmid

#15664

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

USP28 shRNA (USP28 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits
PSUPER-344(hPot1)

Plasmid

#11133

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi

Expression

Mammalian

Mutation

N/A

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pSUPER.retro-{beta}4 siRNA

Plasmid

#16266

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SIRNA Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only