Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 24,746 results for: CHI

Clear keywords
Showing: 11451 - 11500 of 24746 results

  14. mE-mCherry2A

    Plasmid
    #236248
    Purpose
    Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    mCherry2A
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence to mCherry with no stop …
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236248-plasmid-map-sequence-id-486041

    Enlarge

  15. mE-GFP2A

    Plasmid
    #236247
    Purpose
    Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately
    Depositor
    James Amatruda, Genevieve Kendall
    Article
    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10
    Insert
    eGFP
    Expression
    Mammalian
    Mutation
    Added a viral2A sequence with no stop codon
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    236247-plasmid-map-sequence-id-486040

    Enlarge

  16. pUC19_21 tRNA genes

    Plasmid
    #242430
    Purpose
    In vitro transcription of 21 tRNAs after Nt.BspQI treatment. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene.
    Depositor
    Sebastian Maerkl
    Article
    Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:
    Insert
    21 tRNA genes with a T7 promoter upstream and an Nt.BspQI recognition site downstream of each tRNA gene
    Expression
    Bacterial
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    242430-plasmid-map-sequence-id-485004

    Enlarge

  17. SAGA046

    Plasmid
    #240795
    Purpose
    Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA pBR322ROP His medium : T7- His tags medium- gfp -pBR322ROP tetApBR322ROP - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240795-plasmid-map-sequence-id-484228

    Enlarge

  18. SAGA045

    Plasmid
    #240794
    Purpose
    Plasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA pBR322ROP His low : T7- His tags low - gfp -pBR322ROP tetApBR322ROP - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240794-plasmid-map-sequence-id-484225

    Enlarge

  19. SAGA043

    Plasmid
    #240792
    Purpose
    Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA p15a His medium : T7- His tags medium- gfp -p15a tetAp15a - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240792-plasmid-map-sequence-id-484216

    Enlarge

  20. SAGA042

    Plasmid
    #240791
    Purpose
    Plasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA p15a His low : T7- His tags low - gfp -p15a tetAp15a - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240791-plasmid-map-sequence-id-484215

    Enlarge

  21. SAGA040

    Plasmid
    #240789
    Purpose
    Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 His medium : T7- His tags medium- gfp -RK2 tetARK2 - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240789-plasmid-map-sequence-id-484214

    Enlarge

  23. SAGA039

    Plasmid
    #240788
    Purpose
    Plasmid contains the triple selection cassette gfp -RK2 tetARK2 - CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA RK2 His low : T7- His tags low - gfp -RK2 tetARK2 - CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240788-plasmid-map-sequence-id-484210

    Enlarge

  24. pNS1D-sgRNA9-SpR

    Plasmid
    #191092
    Purpose
    aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002
    Depositor
    Anne Ruffing
    Article
    Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    tet
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    191092-plasmid-map-sequence-id-373360

    Enlarge

  34. POC1550

    Plasmid
    #226497
    Purpose
    pMOBC360_VM_C1 assembled plasmid (Chloramphenicol R) containing four inserts (POC1546-POC1549) assembled in POC1525 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226497-plasmid-map-sequence-id-465770

    Enlarge

  35. POC1546

    Plasmid
    #226487
    Purpose
    pMOBK360_VL_N1 assembled plasmid (Kanamycin R) containing four inserts (POC1535-POC1538) assembled in POC1518 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    ssembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226487-plasmid-map-sequence-id-469009

    Enlarge

  36. POC1547

    Plasmid
    #226489
    Purpose
    pMOBK360_VM_N2 assembled plasmid (Kanamycin R) containing three inserts (POC1539-POC1541) assembled in POC1519 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226489-plasmid-map-sequence-id-468559

    Enlarge

  37. POC1548

    Plasmid
    #226490
    Purpose
    pMOBK360_VM_N3 assembled plasmid (Kanamycin R) containing two inserts (POC1542-POC1543) assembled in POC1519 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226490-plasmid-map-sequence-id-468573

    Enlarge

  38. POC1549

    Plasmid
    #226491
    Purpose
    pMOBK360_VR_N4 assembled plasmid (Kanamycin R) containing two inserts (POC1544-POC1545) assembled in POC1520 using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    Expression
    Bacterial
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226491-plasmid-map-sequence-id-465763

    Enlarge

  39. POC1431

    Plasmid
    #221578
    Purpose
    Assembled plasmid containing four inserts (from POC1426, POC1427, POC1428 and POC1429) assembled using the site-selective methylation protection approach
    Depositor
    Christopher A. O'Callaghan
    Article
    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk
    Insert
    Assembled construct flanked by methylation-protectable BsaI sites and containing four always-methylable internal BsaI sites
    Use
    Synthetic Biology
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    221578-plasmid-map-sequence-id-465738

    Enlarge

Showing: 11451 - 11500 of 24746 results