We narrowed to 13,836 results for: CIT

1. pET28a LZ LED Y551A-FLAG

   Plasmid

   #199680

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED Y551A (GPR155 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Y551 of LYCHOS mutated to A

   Promoter

   T7 promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EGFP.T2A.NCLX.3'UTR

   Plasmid

   #181872

   Purpose

   Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Inserts

   solute carrier family 8 member B1 (Slc8b1 Mouse)
   EGFP
   3'UTR of solute carrier family 8 member B1
   

   Use

   AAV and Adenoviral

   Tags

   HA, T2A, and myc

   Expression

   Mammalian

   Mutation

   includes a large portion of the Slc8b1 3'UTR…

   Promoter

   synthetic hybrid CAG promoter

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pαH-S-GSAS-B.1.351.v4

   Plasmid

   #173788

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Venus-BimEL(Noxa)-pEGFP-C1

   Plasmid

   #166761

   Purpose

   To examine effect of swapping the BH3 region in full length BimEL with that of Noxa

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   BimEL(Mus musculus)-(Noxa BH3) (Bcl2l11, PMAIP1 Human, Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   BH3 region removed and inserted BH3 of human Noxa…

   Promoter

   CMV

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lentiviral nontargeting shRNA GFP

   Plasmid

   #155285

   Purpose

   Lentiviral expression of nontargeting shRNA, GFP expression, based on Addgene 12247

   Depositor

   Martin Carroll

   Insert

   Nontargeting shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dox nontargeting shRNA GFP

   Plasmid

   #155290

   Purpose

   Lentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652

   Depositor

   Martin Carroll

   Insert

   Nontargeting shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dox nontargeting shRNA mKate

   Plasmid

   #155292

   Purpose

   Lentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652

   Depositor

   Martin Carroll

   Insert

   Nontargeting shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lentiviral nontargeting shRNA RFP i670

   Plasmid

   #155287

   Purpose

   Lentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247

   Depositor

   Martin Carroll

   Insert

   Nontargeting shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH200

   Plasmid

   #13327

   Depositor

   Martin Gellert

   Article

   Lieber et al Genes Dev. 1987 Oct . 1(8):751-61.

   Insert

   recombination substrate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pJK241

    Plasmid

    #71693

    Purpose

    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)

    Depositor

    T. J. Kappock

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed Tro404 to Phe

    Promoter

    T7 promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK699

    Plasmid

    #71691

    Purpose

    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)

    Depositor

    T. J. Kappock

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed Asp371 to Asn

    Promoter

    T7 promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK708

    Plasmid

    #71692

    Purpose

    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)

    Depositor

    T. J. Kappock

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed Trp400 to Phe

    Promoter

    T7 promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid

    #62723

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1 and AAV5

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1α1.1-ChrimsonR-tdTomato

    Plasmid

    #122064

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1a（1.1kb short version)

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJOP-HTT-HR65Q

    Plasmid

    #92249

    Purpose

    HTT donor DNA with 65Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJOP-HTT-HR81Q

    Plasmid

    #92250

    Purpose

    HTT donor DNA with 81Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJOP-HTT-HR30Q

    Plasmid

    #92247

    Purpose

    HTT donor DNA with 30Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-KrasG12D/sgKras/Cre

    Plasmid

    #99851

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid

    #154072

    Purpose

    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Inserts

    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    

    Use

    Tags

    GST, His6, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJOP-HTT-HR45Q

    Plasmid

    #92248

    Purpose

    HTT donor DNA with 45Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge