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TGIF2 A11.3 gRNA

Plasmid

#90910

Purpose

3rd generation lentiviral gRNA plasmid targeting human TGIF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TGIF2 (Guide Designation A11.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(WT)

Plasmid

#112720

Purpose

Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(WT)

Tags

6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48T)

Plasmid

#112725

Purpose

This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48T)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48T

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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PAK4-3'UTR-BS1

Plasmid

#113090

Purpose

Luciferase reporter containing PAK4 3' UTR with the first predicted MiR-199a-3p binding site

Depositor

James Donahue

Article

Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840

Insert

p21 Activated kinase 4 (PAK4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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TTK H10.1 gRNA

Plasmid

#90923

Purpose

3rd generation lentiviral gRNA plasmid targeting human TTK

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TTK (Guide Designation H10.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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NDE1 F1.2 gRNA

Plasmid

#90788

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDE1 (Guide Designation F1.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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AURKA A1.1 gRNA

Plasmid

#90540

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKA (Guide Designation A1.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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CKS2 C8.2 gRNA

Plasmid

#90633

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKS2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CKS2 (Guide Designation C8.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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CCNA1 C8.5 gRNA

Plasmid

#90573

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCNA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCNA1 (Guide Designation C8.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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H2AFZ B2.3 gRNA

Plasmid

#90705

Purpose

3rd generation lentiviral gRNA plasmid targeting human H2AFZ

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

H2AFZ (Guide Designation B2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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KIF4A D10.2 gRNA

Plasmid

#90725

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF4A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF4A (Guide Designation D10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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UNG D11.3 gRNA

Plasmid

#90936

Purpose

3rd generation lentiviral gRNA plasmid targeting human UNG

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

UNG (Guide Designation D11.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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MCM6 E2.2 gRNA

Plasmid

#90763

Purpose

3rd generation lentiviral gRNA plasmid targeting human MCM6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MCM6 (Guide Designation E2.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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ACTR1B C11.5 gRNA

Plasmid

#90524

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACTR1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ACTR1B (Guide Designation C11.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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AURKA A2.4 gRNA

Plasmid

#90543

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKA

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKA (Guide Designation A2.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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PCNA G4.1 gRNA

Plasmid

#90829

Purpose

3rd generation lentiviral gRNA plasmid targeting human PCNA

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PCNA (Guide Designation G4.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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CENPA B11.1 gRNA

Plasmid

#90610

Purpose

3rd generation lentiviral gRNA plasmid targeting human CENPA

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CENPA (Guide Designation B11.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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