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Plasmid#65683PurposeExpresses Zyxin-ONL in mammalian cellsDepositorInsertzyxin (Zyx Mouse)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_3xNLS
Plasmid#65674PurposeExpresses NLS-ONL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_TUBB5
Plasmid#65680PurposeExpresses beta-tubulin-ONL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLuc-His10
Plasmid#234567PurposeBacterially expressed NLuc for Ni-NTA purificationDepositorInsertNano luciferase
UseTagsT7 gene 10 leader peptide and Thrombin site-10xHi…ExpressionBacterialMutationCodon optimized for bacterial expressionPromoterT7Available sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICH47811_AvrSr62-7
Plasmid#233534PurposeExpresses AvrSr62-7 in protoplastsDepositorInsertAvrSr62-7
UseProtoplast expressionTagsLuciferaseExpressionPlantMutationPromoterUbiquitinAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-1_Rluc-Y39x-Fluc
Plasmid#233615PurposeExpression of Rluc-Fluc reporter gene containing 39xYDepositorInsertRluc-Y39x-Fluc
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pp-CBBC-Fluc
Plasmid#232920PurposeAllows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBK_Gal4-DBD-ESR1
Plasmid#227099PurposeESR1 receptor overexpression for luciferase and barcode assaysDepositorInsertESR1 (ESR1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-GFPenhancer
Plasmid#202468PurposeExpresses the nanobody GFP enhancer after phosphate depletionDepositorInsertnanobody GFP enhancer
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-GFPminimizer
Plasmid#202469PurposeExpresses the nanobody GFP minimizer after phosphate depletionDepositorInsertnanobody GFP minimizer
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-roGFP2
Plasmid#202462PurposeExpresses redox sensitive GFP (roGFP) after phosphate depletionDepositorInsertreduction-oxidation sensitive green fluorescent protein
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM154
Plasmid#218121PurposeHybrid circuit displaying FKBP12 bait and driving expression of luxABDepositorInsertsFKBP12
luxAB
UseLuciferaseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROF021
Plasmid#155326PurposeExpression of PULSE-controlled FLucDepositorInsert(etr)8-(C120)5-PhCMVmin-FLuc-TSV40
UseLuciferase and Synthetic Biology; Eukaryotic expr…TagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D3:Luc
Plasmid#192990PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D3PromoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D1:Luc
Plasmid#192988PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D1PromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D2:Luc
Plasmid#192989PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D2PromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep-D4:Luc
Plasmid#192991PurposePlasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Wnt3 repressor element-D4PromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3-ΔRep:Luc
Plasmid#192987PurposePlasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)DepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationRepressor element deletedPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2h70CRET(#325)
Plasmid#184060Purposecyclofen-inducible CRE activation in zebrafish transient transgenicDepositorInsertCRE-ERT2
UseZebrafish transient transgenesisTagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Hsp70-4Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Ala2
Plasmid#71326PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterinsulated waaHp from E. coliAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only