We narrowed to 32,113 results for: KIN

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904 pLNCX HA Akt1 K179M

Plasmid

#9007

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K179M. Kinase dead.

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pBrain-GFP-FKBP-TACC3-shTACC3

Plasmid

#59354

Purpose

Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Insert

Transforming Acidic Coiled Coil protein 3 (TACC3 Human)

Use

RNAi

Tags

EGFP and FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4 K41M-3xFLAG

Plasmid

#69838

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a-GFP-AURKA_K162M-mCherry

Plasmid

#99881

Purpose

Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP and mCherry

Expression

Bacterial

Mutation

K162M (kinase-dead)

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP gamma

Plasmid

#12560

Depositor

Peter Johnson

Article

Parkin et al J Biol Chem. 2002 Jun 28;277(26):23563-7

Insert

CCAAT enhancer binding protein gamma (Cebpg Mouse)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST JNKKTR AA Clover

Plasmid

#90238

Purpose

Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AA mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KA

Plasmid

#133582

Purpose

Expresses IP3KA GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only
pGP18 (CK2alpha' K69M)

Plasmid

#27090

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' K69M (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Mutation

K69M

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
GST-PS-Grb2 1-154 (XSB382)

Plasmid

#78510

Purpose

Construct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 1-154 (GRB2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FAM20C:V5

Plasmid

#63584

Purpose

FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.

Depositor

Kenneth Irvine

Article

Ishikawa et al PLoS One. 2012;7(8):e42988. doi: 10.1371

Insert

FAM20C (Fam20c Mouse)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pAB07 (CK2beta)

Plasmid

#27091

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Tags

Myc

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-2xmCitrine-GFP11

Plasmid

#61667

Purpose

Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK5

Plasmid

#53166

Purpose

Expresses GST-HA tagged human MEK5 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Tags

GST and HA

Expression

Bacterial

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR EE Clover

Plasmid

#90239

Purpose

Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (EE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DYRK2-2A-mCherry

Plasmid

#118285

Purpose

Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Promoter

CMV with beta global intron

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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JpExpress404 PTEN

Plasmid

#49420

Purpose

Bacterial Expression Vector

Depositor

Ramon Parsons

Article

Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

Insert

PTEN (PTEN )

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-DYRK3-2A-mCherry

Plasmid

#118287

Purpose

Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Promoter

CMV with beta global intron

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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hSyn1-AsCpf1(RR)

Plasmid

#109325

Purpose

AAV vector expressing AsCpf1 (RR variant)

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

hAsCpf1(RR)

Use

AAV

Tags

HA and NLS

Mutation

S542R/K607R

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KC

Plasmid

#134603

Purpose

Expresses IP3KC GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KC (ITPKC Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA Myc-mMEKK2

Plasmid

#44158

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

Mekk2 (Map3k2 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only