We narrowed to 5,636 results for: chia

1. Traptavidin-E6

   Plasmid

   #59549

   Purpose

   pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Core Traptavidin-E6
   

   Use

   Tags

   E6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Skp

   Plasmid

   #213139

   Purpose

   Expresses chaperone protein SKP

   Depositor

   Sebastian Hiller

   Article

   Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

   Insert

   SKP (21-161) (skp E.coli)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_T7-ARG2

   Plasmid

   #106474

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAR357_SFP_pCDF-1b

   Plasmid

   #122851

   Purpose

   Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   Sfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOxyR_rfp

   Plasmid

   #194155

   Purpose

   Red Fluorescent Reporter of intracellular hydrogen peroxide

   Depositor

   Sven Panke

   Article

   Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

   Insert

   transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PoxyR, PoxyS

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas3

   Plasmid

   #229538

   Purpose

   pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Insert

   Cas3
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLS.400

   Plasmid

   #187201

   Purpose

   Expresses retron Eco4 RT

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Insert

   Retron Eco4 Reverse Transcriptase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   mphR

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMART-SOS-GFPuv

   Plasmid

   #102283

   Purpose

   Fluorescent sensor of E. coli SOS response

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   cda-GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cda SOS promoter

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. SSB-wt

   Plasmid

   #197260

   Purpose

   wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

   Depositor

   Timothy Lohman

   Article

   Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

   Insert

   wt E. coli SSB
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p006-GFP-Logic-AND

    Plasmid

    #112237

    Purpose

    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Inserts

    GFP under control of doubly-repressible promoter
    GFP
    araC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AND-gate and pLacI

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)

    Plasmid

    #199763

    Purpose

    Elongation reporter construct to quantify elongation of LacZ

    Depositor

    Brian Zid

    Article

    Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

    Insert

    LacZ-nLucPEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRT802

    Plasmid

    #68113

    Purpose

    Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.

    Depositor

    Maggie Smith

    Article

    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

    Inserts

    aac3(IV)
    aph11
    oriT
    attP
    int
    

    Use

    Streptomyces integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMPTY::sgRNA2

    Plasmid

    #165459

    Purpose

    Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

    Depositor

    Inigo Lasa

    Article

    Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

    Insert

    sgRNA2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP01

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLS.491

    Plasmid

    #184956

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C

    Promoter

    T7/lac

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXD70LacZ2-Pdadh-LacZ

    Plasmid

    #191618

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ABE8r-V106W

    Plasmid

    #208295

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-V106W-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3xFlag-Dam

    Plasmid

    #121909

    Purpose

    Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Dam human codon optimized (dam E. coli)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-subtilase A

    Plasmid

    #35948

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    Subtilase A (subA E. Coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

20. ABE8r-R153del

    Plasmid

    #208296

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-R153del-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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