We narrowed to 35,675 results for: lat
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Plasmid#65107PurposeExpression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cellsDepositorInsertDelta(Pro-MP)ADAM10 (ADAM10 Human)
TagsHAExpressionMammalianMutationdeleted AA 19-455PromoterCMVAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP5 (catalytic domain)
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
TagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-3HA-SREBP1-C-Flag
Plasmid#134286PurposeLentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1DepositorInsertSREBP1 (Srebf1 Mouse)
UseLentiviralTags3x HA and FlagExpressionMammalianMutationmouse SREBP1 isoform a precursorPromoterEF1aAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pERM2-nhpSer
Plasmid#201922PurposeEnables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.DepositorInsertsSepRS(2)
Sep-tRNA v2.0
SerB
EF-Sep
TagsNoneExpressionBacterialMutationE412P, E414F, T417K, P495M, I496W, F529S and H67R…PromoterGlnS (constitutive), OXB20 (constitutive), lpp (c…Available SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMVNeoDEST(705-1)-hAXL
Plasmid#124796PurposeExpresses AXL in mammalian cellsDepositorAvailable SinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN ltDHFR(DD)-YFP
Plasmid#47076PurposeecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegansDepositorInsertltDHFR(DD)
UseRetroviralTagsHA and YFPMutationamino acids mutations: R12H, N23S, G67S, V78A, E1…PromoterLTRAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAB07 (CK2beta)
Plasmid#27091DepositorAvailable SinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CD74-ROS1 nonmutant
Plasmid#183813PurposeExpress CD74-ROS1 fusion (C6;R34) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of CD74 exon 1-6 and ROS1 exon…ExpressionMammalianPromoterCMVAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2-P2A-BSD
Plasmid#174818PurposeMammalian expression of SaCas9 prime editor 2 with P2A-BSD markerDepositorInsertSaPE2-P2A-BSD
TagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSNCA-NE
Plasmid#102361PurposeOverexpression of synuclein alpha in mammalian cellsDepositorAvailable SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAGEdeltaCyto
Plasmid#71436Purposemammalian expression of human RAGE cytoplasmic domain mutantDepositorAvailable SinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP-shTACC3
Plasmid#59356PurposeKnocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsGFPExpressionMammalianMutationSilent mutations in amino acids 30-33 to confer s…PromoterCMVAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-X-GFP
Plasmid#58245PurposeGATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFPDepositorInsertsattR1-Cm(R)-CcdB-attR2
TetR-NLS-T2A-GFP
UseLentiviral and RNAi; Gateway destionation vectorTagsGFP, NLS, T2A, and TetRExpressionMammalianPromoterSFFV and noAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 EWS-myc-HIS
Plasmid#46386Purposeexpresses myc, HIS tagged EWSR1 in mammalian cellsDepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-GFP
Plasmid#66083PurposeG protein alpha-s internally tagged with EGFP and EE epitopeDepositorInsertG-alpha-s-EE-EGFP (Gnas Rat)
TagsEGFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga13
Plasmid#196062PurposeEncodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE
Plasmid#202199PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombinationDepositorHas ServiceAAV1 and AAV5InsertstChrimsonR, PdCO
UseAAV and Cre/LoxTagsEGFP and Rho1D4ExpressionMammalianPromoterhSyn1Available SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Neo GFP-Progerin C661S
Plasmid#118711PurposeLentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)DepositorInsertGFP-Progerin C661S (LMNA Human)
UseLentiviral; Tet-on inducibleTagsGFPExpressionMammalianMutationC661S farnesylation mutantPromoterCMV TRE3G (TET-ON)Available SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mitoGFP-DIO
Plasmid#174112PurposeCre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoterDepositorHas ServiceAAV2InsertmitoGFP (COX8A Human)
UseAAVTagsCox8 targeting sequence and GFPPromoterEf1aAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_GCaMP6f
Plasmid#213943PurposeAAV construct expressing GCaMP6f driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_GCaMP6fDepositorHas ServiceAAV PHP.eB and AAV1InsertGCaMP6f
UseAAVAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only